Author
Listed:
- Kotaro Nakanishi
(Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa 225-8501, Japan
Present address: Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10065, USA.)
- Luc Bonnefond
(Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan)
- Satoshi Kimura
(Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan)
- Tsutomu Suzuki
(Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan)
- Ryuichiro Ishitani
(Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan)
- Osamu Nureki
(Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa 225-8501, Japan
Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan)
Abstract
Translational fidelity Transfer RNAs are transcribed as precursor molecules which are then shortened, have a short sequence added, and can undergo recoding of certain nucleotides to generate a different amino acid specificity. In this work, Nakanishi et al. show how the bacterial enzyme TilS uniquely recognizes a precursor tRNA that has a specificity for methionine and then introduces a lysidine-for-cytidine modification in the anticodon that results in the mature tRNA coding for isoleucine. They find that by acting upon the precursor tRNA, TilS thereby prevents charging of the tRNA by the methionyl-tRNA synthetase, which only acts on a mature tRNA. This mechanism contributes to the vital goal of maintaining the fidelity of isoleucine codon translation in bacteria.
Suggested Citation
Kotaro Nakanishi & Luc Bonnefond & Satoshi Kimura & Tsutomu Suzuki & Ryuichiro Ishitani & Osamu Nureki, 2009.
"Structural basis for translational fidelity ensured by transfer RNA lysidine synthetase,"
Nature, Nature, vol. 461(7267), pages 1144-1148, October.
Handle:
RePEc:nat:nature:v:461:y:2009:i:7267:d:10.1038_nature08474
DOI: 10.1038/nature08474
Download full text from publisher
As the access to this document is restricted, you may want to search for a different version of it.
Corrections
All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:461:y:2009:i:7267:d:10.1038_nature08474. See general information about how to correct material in RePEc.
If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.
We have no bibliographic references for this item. You can help adding them by using this form .
If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.
For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .
Please note that corrections may take a couple of weeks to filter through
the various RePEc services.