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Gal4 turnover and transcription activation

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  • Galen A. Collins

    (Watson School of Biological Sciences, 1 Bungtown Road
    Cold Spring Harbor Laboratory, 1 Bungtown Road
    Present addresses: Vanderbilt University Medical Centre, 465 21st Avenue South, Nashville, Tennessee 37232, USA (G.A.C. and W.P.T.); Amgen, 1 Amgen Center Drive, Thousand Oaks, California 91320-1799, USA (J.R.L.))

  • J. Russell Lipford

    (‡ Caltech, 1200 East California Boulevard, Pasadena, California 91125, USA
    Present addresses: Vanderbilt University Medical Centre, 465 21st Avenue South, Nashville, Tennessee 37232, USA (G.A.C. and W.P.T.); Amgen, 1 Amgen Center Drive, Thousand Oaks, California 91320-1799, USA (J.R.L.))

  • Raymond J. Deshaies

    (‡ Caltech, 1200 East California Boulevard, Pasadena, California 91125, USA)

  • William P. Tansey

    (Cold Spring Harbor Laboratory, 1 Bungtown Road
    Present addresses: Vanderbilt University Medical Centre, 465 21st Avenue South, Nashville, Tennessee 37232, USA (G.A.C. and W.P.T.); Amgen, 1 Amgen Center Drive, Thousand Oaks, California 91320-1799, USA (J.R.L.))

Abstract

Arising from: K. Nalley, S. A. Johnston & T. Kodadek Nature 442, 1054–1057 (2006)10.1038/nature05067 ; Nalley et al. reply Growing evidence supports the notion that proteasome-mediated destruction of transcriptional activators can be intimately coupled to their function1,2. Recently, Nalley et al.3 challenged this view by reporting that the prototypical yeast activator Gal4 does not dynamically associate with chromatin, but rather ‘locks in’ to stable promoter complexes that are resistant to competition. Here we present evidence that the assay used to reach this conclusion is unsuitable, and that promoter-bound, active Gal4 is indeed susceptible to competition in vivo. Our data challenge the key evidence that Nalley et al.3 used to reach their conclusion, and indicate that Gal4 functions in vivo within the context of dynamic promoter complexes.

Suggested Citation

  • Galen A. Collins & J. Russell Lipford & Raymond J. Deshaies & William P. Tansey, 2009. "Gal4 turnover and transcription activation," Nature, Nature, vol. 461(7265), pages 7-7, October.
    • Handle: RePEc:nat:nature:v:461:y:2009:i:7265:d:10.1038_nature08406
    DOI: 10.1038/nature08406
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