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Direct RNA sequencing

Author

Listed:
  • Fatih Ozsolak

    (Helicos BioSciences Corporation, One Kendall Square, Cambridge, Massachusetts 02139, USA)

  • Adam R. Platt

    (Helicos BioSciences Corporation, One Kendall Square, Cambridge, Massachusetts 02139, USA)

  • Dan R. Jones

    (Helicos BioSciences Corporation, One Kendall Square, Cambridge, Massachusetts 02139, USA)

  • Jeffrey G. Reifenberger

    (Helicos BioSciences Corporation, One Kendall Square, Cambridge, Massachusetts 02139, USA)

  • Lauryn E. Sass

    (Helicos BioSciences Corporation, One Kendall Square, Cambridge, Massachusetts 02139, USA)

  • Peter McInerney

    (Helicos BioSciences Corporation, One Kendall Square, Cambridge, Massachusetts 02139, USA)

  • John F. Thompson

    (Helicos BioSciences Corporation, One Kendall Square, Cambridge, Massachusetts 02139, USA)

  • Jayson Bowers

    (Helicos BioSciences Corporation, One Kendall Square, Cambridge, Massachusetts 02139, USA)

  • Mirna Jarosz

    (Helicos BioSciences Corporation, One Kendall Square, Cambridge, Massachusetts 02139, USA)

  • Patrice M. Milos

    (Helicos BioSciences Corporation, One Kendall Square, Cambridge, Massachusetts 02139, USA)

Abstract

A direct line to the transcriptome Understanding the functional output of the genome — the sum total of messenger RNAs in a cell or cell population, known as the transcriptome — is an essential step on the way to understanding biology. Current methods for studying the transcriptome rely on microarray and sequencing approaches that require complementary DNA synthesis followed by multiple manipulations, which introduce biases and potential artefacts. Now a team based at Helicos BioSciences Corporation has developed a direct single-molecule RNA sequencing technique that when scaled-up promises a bias-free high-throughput transcriptome analysis.

Suggested Citation

  • Fatih Ozsolak & Adam R. Platt & Dan R. Jones & Jeffrey G. Reifenberger & Lauryn E. Sass & Peter McInerney & John F. Thompson & Jayson Bowers & Mirna Jarosz & Patrice M. Milos, 2009. "Direct RNA sequencing," Nature, Nature, vol. 461(7265), pages 814-818, October.
    • Handle: RePEc:nat:nature:v:461:y:2009:i:7265:d:10.1038_nature08390
    DOI: 10.1038/nature08390
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