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Proteome-wide cellular protein concentrations of the human pathogen Leptospira interrogans

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  • Johan Malmström

    (Institute of Molecular Systems Biology, ETH Zurich (Swiss Federal Institute of Technology), Wolfgang Pauli-Strasse 16, CH-8093 Zurich, Switzerland)

  • Martin Beck

    (Institute of Molecular Systems Biology, ETH Zurich (Swiss Federal Institute of Technology), Wolfgang Pauli-Strasse 16, CH-8093 Zurich, Switzerland)

  • Alexander Schmidt

    (Institute of Molecular Systems Biology, ETH Zurich (Swiss Federal Institute of Technology), Wolfgang Pauli-Strasse 16, CH-8093 Zurich, Switzerland
    Competence Center for Systems Physiology and Metabolic Diseases)

  • Vinzenz Lange

    (Institute of Molecular Systems Biology, ETH Zurich (Swiss Federal Institute of Technology), Wolfgang Pauli-Strasse 16, CH-8093 Zurich, Switzerland
    Competence Center for Systems Physiology and Metabolic Diseases)

  • Eric W. Deutsch

    (Institute for Systems Biology, 1441 North 34th Street, Seattle, Washington 98103-8904, USA)

  • Ruedi Aebersold

    (Institute of Molecular Systems Biology, ETH Zurich (Swiss Federal Institute of Technology), Wolfgang Pauli-Strasse 16, CH-8093 Zurich, Switzerland
    Competence Center for Systems Physiology and Metabolic Diseases
    Institute for Systems Biology, 1441 North 34th Street, Seattle, Washington 98103-8904, USA
    Faculty of Science, University of Zurich)

Abstract

Proteins by numbers Absolute protein quantification via the measurement of protein copy numbers has the potential to provide important information about biological processes, but apart from the special case of brewers yeast, this has not proved possible using standard proteomics procedures. Now, with the leptospirosis pathogen Leptospira interrogans as the first target, a new mass spectrometry-based strategy that should be generally applicable to many other biological systems has been used to determine absolute protein abundance for a significant fraction of a proteome. The results show how L. interrogans adapts to changing environments by adjusting proteome homeostasis while the total protein copy numbers remain constant.

Suggested Citation

  • Johan Malmström & Martin Beck & Alexander Schmidt & Vinzenz Lange & Eric W. Deutsch & Ruedi Aebersold, 2009. "Proteome-wide cellular protein concentrations of the human pathogen Leptospira interrogans," Nature, Nature, vol. 460(7256), pages 762-765, August.
    • Handle: RePEc:nat:nature:v:460:y:2009:i:7256:d:10.1038_nature08184
    DOI: 10.1038/nature08184
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    Cited by:

    1. Fabrizio Clarelli & Adam Palmer & Bhupender Singh & Merete Storflor & Silje Lauksund & Ted Cohen & Sören Abel & Pia Abel zur Wiesch, 2020. "Drug-target binding quantitatively predicts optimal antibiotic dose levels in quinolones," PLOS Computational Biology, Public Library of Science, vol. 16(8), pages 1-27, August.

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