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Cai et al. reply

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  • Chen-Leng Cai

    (* Skaggs School of Pharmacy, University of California, San Diego, La Jolla, California 92093, USA. syevans@ucsd.edu
    Mount Sinai School of Medicine, 1 Gustave L. Levy Place, New York, New York 10029, USA
    ‖ Centre for Molecular Cardiology, Mount Sinai School of Medicine, 1 Gustave L. Levy Place, New York, New York 10029, USA
    ¶ The Black Family Stem Cell Institute, Mount Sinai School of Medicine, 1 Gustave L. Levy Place, New York, New York 10029, USA)

  • Jody C. Martin

    (University of California, San Diego, La Jolla, California 92093, USA)

  • Yunfu Sun

    (* Skaggs School of Pharmacy, University of California, San Diego, La Jolla, California 92093, USA. syevans@ucsd.edu)

  • Li Cui

    (* Skaggs School of Pharmacy, University of California, San Diego, La Jolla, California 92093, USA. syevans@ucsd.edu)

  • Lianchun Wang

    (Complex Carbohydrate Research Centre, University of Georgia)

  • Kunfu Ouyang

    (University of California, San Diego, La Jolla, California 92093, USA)

  • Lei Yang

    (* Skaggs School of Pharmacy, University of California, San Diego, La Jolla, California 92093, USA. syevans@ucsd.edu)

  • Lei Bu

    (* Skaggs School of Pharmacy, University of California, San Diego, La Jolla, California 92093, USA. syevans@ucsd.edu)

  • Xingqun Liang

    (University of California, San Diego, La Jolla, California 92093, USA)

  • Xiaoxue Zhang

    (* Skaggs School of Pharmacy, University of California, San Diego, La Jolla, California 92093, USA. syevans@ucsd.edu)

  • William B. Stallcup

    (Developmental Neurobiology Programme, The Burnham Institute)

  • Christopher P. Denton

    (Centre for Rheumatology, Royal Free and University College Medical School)

  • Andrew McCulloch

    (University of California, San Diego, La Jolla, California 92093, USA)

  • Ju Chen

    (University of California, San Diego, La Jolla, California 92093, USA)

  • Sylvia M. Evans

    (* Skaggs School of Pharmacy, University of California, San Diego, La Jolla, California 92093, USA. syevans@ucsd.edu
    University of California, San Diego, La Jolla, California 92093, USA)

Abstract

Replying to: V. M. Christoffels et al. Nature 458, 10.1038/nature07916 (2009) We recognized the importance of confirming data from Cre lineage studies with an alternative approach, and thus used a fluorescent dye to lineage-label epicardial cells. This approach confirmed that epicardial cells contributed to myocytes within the heart1. In our experiments, we never observed Tbx18 mRNA within ventricles at embryonic day E10.5 to E11.5, although our RNA signal in epicardium at these stages is at least as strong as that observed by Christoffels et al.2. We note a discrepancy between amount of RNA staining (broadly throughout the middle of the ventricle) and protein staining (much more restricted) that Christoffels et al.2 observe at early stages. Reasons for discrepancies between our RNA in situ data and that of Christoffels et al. are unclear. Christoffels et al.2 detect Tbx18 intronic sequences by RT–PCR of E11.5 IVS, but do not provide data to demonstrate mature Tbx18 mRNA. Notably, we did observe active expression of Tbx18 in non-myocytes after E11.5 (ref. 1).

Suggested Citation

  • Chen-Leng Cai & Jody C. Martin & Yunfu Sun & Li Cui & Lianchun Wang & Kunfu Ouyang & Lei Yang & Lei Bu & Xingqun Liang & Xiaoxue Zhang & William B. Stallcup & Christopher P. Denton & Andrew McCulloch , 2009. "Cai et al. reply," Nature, Nature, vol. 458(7240), pages 9-10, April.
    • Handle: RePEc:nat:nature:v:458:y:2009:i:7240:d:10.1038_nature07917
    DOI: 10.1038/nature07917
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