IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v457y2009i7233d10.1038_nature07784.html
   My bibliography  Save this article

Is REST a regulator of pluripotency?

Author

Listed:
  • Noel J. Buckley

    (* King’s College London, Institute of Psychiatry, Centre for the Cellular Basis of Behaviour, James Black Centre, 125 Coldharbour Lane, London SE5 9NU, UK. noel.buckley@iop.kcl.ac.uk)

  • Rory Johnson

    († Stem Cell and Developmental Biology, Genome Institute of Singapore, 60 Biopolis Street #02-01, Genome Building, Singapore 138672)

  • Yuh-Man Sun

    (* King’s College London, Institute of Psychiatry, Centre for the Cellular Basis of Behaviour, James Black Centre, 125 Coldharbour Lane, London SE5 9NU, UK. noel.buckley@iop.kcl.ac.uk)

  • Lawrence W. Stanton

    († Stem Cell and Developmental Biology, Genome Institute of Singapore, 60 Biopolis Street #02-01, Genome Building, Singapore 138672)

Abstract

Arising from: S. K. Singh, M. N. Kagalwala, J. Parker-Thornburg, H. Adams & S. Majumder Nature 453, 223–227 (2008)10.1038/nature06863 ; Singh et al. reply Establishment and maintenance of the pluripotent state of ESCs is a key issue in stem cell biology and regenerative medicine, and consequently identification of transcription factors that regulate ESC pluripotency is an important goal. Singh et al.1 claim that the transcriptional repressor REST is such a regulator and that a 50% reduction of REST in ESCs leads to activation of a specific microRNA, miR-21, and that this subsequently results in loss of pluripotency markers and a reciprocal gain in some lineage-specific differentiation markers. In contrast, we show that, in haplodeficient Rest+/- ESCs, we detected no change in pluripotency markers, no precocious expression of differentiated neuronal markers and no interaction of REST with miR-21. It is vital that identification of factors that regulate pluripotency is based on robust, consistent data, and the contrast in data reported here undermines the claim by Singh et al.1 that REST is such a regulator.

Suggested Citation

  • Noel J. Buckley & Rory Johnson & Yuh-Man Sun & Lawrence W. Stanton, 2009. "Is REST a regulator of pluripotency?," Nature, Nature, vol. 457(7233), pages 5-6, February.
    • Handle: RePEc:nat:nature:v:457:y:2009:i:7233:d:10.1038_nature07784
    DOI: 10.1038/nature07784
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature07784
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature07784?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:457:y:2009:i:7233:d:10.1038_nature07784. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.