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Endonucleolytic RNA cleavage by a eukaryotic exosome

Author

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  • Alice Lebreton

    (Equipe Labellisée La Ligue, Centre de Génétique Moléculaire, CNRS UPR 2167, 91198 Gif-sur-Yvette, France
    Université Paris-Sud
    Université Pierre et Marie Curie-Paris 6)

  • Rafal Tomecki

    (Faculty of Biology, University of Warsaw
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences)

  • Andrzej Dziembowski

    (Faculty of Biology, University of Warsaw
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences)

  • Bertrand Séraphin

    (Equipe Labellisée La Ligue, Centre de Génétique Moléculaire, CNRS UPR 2167, 91198 Gif-sur-Yvette, France
    Université Paris-Sud
    Université Pierre et Marie Curie-Paris 6)

Abstract

RNA processing: a dual role exosome The exosome is a multisubunit exonuclease complex that degrades many types of RNAs, in many different contexts, in a 3' to 5' manner. The catalytic component of the exosome is the Dis3 subunit. Dis3 contains a PIN domain, which is sometimes associated with nuclease activity. In this work, Lebreton et al. show that the Dis3 PIN domain possesses endonuclease activity (that is, it can cleave RNA internally, rather than from an end). Mutations in either this domain or in the exonuclease domain exhibit a growth phenotype, suggesting that both activities are physiologically important. While other RNase complexes contain both endo- and exonuclease activities, this is the first example of both activities being encoded in a single protein.

Suggested Citation

  • Alice Lebreton & Rafal Tomecki & Andrzej Dziembowski & Bertrand Séraphin, 2008. "Endonucleolytic RNA cleavage by a eukaryotic exosome," Nature, Nature, vol. 456(7224), pages 993-996, December.
    • Handle: RePEc:nat:nature:v:456:y:2008:i:7224:d:10.1038_nature07480
    DOI: 10.1038/nature07480
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