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Generation of pluripotent stem cells from adult human testis

Author

Listed:
  • Sabine Conrad

    (Institute of Anatomy)

  • Markus Renninger

    (University Clinic Tübingen, Hoppe-Seyler-Straße 3, Tübingen 72076, Germany)

  • Jörg Hennenlotter

    (University Clinic Tübingen, Hoppe-Seyler-Straße 3, Tübingen 72076, Germany)

  • Tina Wiesner

    (Institute of Anatomy)

  • Lothar Just

    (Institute of Anatomy)

  • Michael Bonin

    (Institute of Anthropology and Human Genetics, Microarray Facility, University Clinic, Calwerstraße 7, 72076 Tübingen, Germany)

  • Wilhelm Aicher

    (ZMF Research Laboratories, University Clinic Tübingen, Waldhörnlestraße 22, 72072 Tübingen, Germany
    Center for Regenerative Biology and Medicine (ZRM), Paul-Ehrlich-Straße 15, 72076 Tübingen, Germany)

  • Hans-Jörg Bühring

    (University Clinic Tübingen, Otfried-Müller-Straße 10, 72076 Tübingen, Germany)

  • Ulrich Mattheus

    (Institute of Anatomy, Österbergstraße 3, 72074 Tübingen, Germany)

  • Andreas Mack

    (Institute of Anatomy, Österbergstraße 3, 72074 Tübingen, Germany)

  • Hans-Joachim Wagner

    (Institute of Anatomy, Österbergstraße 3, 72074 Tübingen, Germany)

  • Stephen Minger

    (Stem Cell Biology Laboratory, Wolfson Centre for Age-Related Diseases, King's College London, King's College)

  • Matthias Matzkies

    (Institute of Neurophysiology, University of Cologne, Robert-Koch-Straße 39, 50931 Cologne, Germany)

  • Michael Reppel

    (Institute of Neurophysiology, University of Cologne, Robert-Koch-Straße 39, 50931 Cologne, Germany)

  • Jürgen Hescheler

    (Institute of Neurophysiology, University of Cologne, Robert-Koch-Straße 39, 50931 Cologne, Germany)

  • Karl-Dietrich Sievert

    (University Clinic Tübingen, Hoppe-Seyler-Straße 3, Tübingen 72076, Germany)

  • Arnulf Stenzl

    (University Clinic Tübingen, Hoppe-Seyler-Straße 3, Tübingen 72076, Germany)

  • Thomas Skutella

    (Institute of Anatomy
    Center for Regenerative Biology and Medicine (ZRM), Paul-Ehrlich-Straße 15, 72076 Tübingen, Germany)

Abstract

Human primordial germ cells and mouse neonatal and adult germline stem cells are pluripotent and show similar properties to embryonic stem cells. Here we report the successful establishment of human adult germline stem cells derived from spermatogonial cells of adult human testis. Cellular and molecular characterization of these cells revealed many similarities to human embryonic stem cells, and the germline stem cells produced teratomas after transplantation into immunodeficient mice. The human adult germline stem cells differentiated into various types of somatic cells of all three germ layers when grown under conditions used to induce the differentiation of human embryonic stem cells. We conclude that the generation of human adult germline stem cells from testicular biopsies may provide simple and non-controversial access to individual cell-based therapy without the ethical and immunological problems associated with human embryonic stem cells.

Suggested Citation

  • Sabine Conrad & Markus Renninger & Jörg Hennenlotter & Tina Wiesner & Lothar Just & Michael Bonin & Wilhelm Aicher & Hans-Jörg Bühring & Ulrich Mattheus & Andreas Mack & Hans-Joachim Wagner & Stephen , 2008. "Generation of pluripotent stem cells from adult human testis," Nature, Nature, vol. 456(7220), pages 344-349, November.
    • Handle: RePEc:nat:nature:v:456:y:2008:i:7220:d:10.1038_nature07404
    DOI: 10.1038/nature07404
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