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Does Rft1 flip an N-glycan lipid precursor?

Author

Listed:
  • Christian G. Frank

    (Weill Cornell Medical College
    †Present address: Fundación Caubet-CIMERA, 07110 Bunyola, Mallorca, Illes Balears, Spain)

  • Sumana Sanyal

    (Weill Cornell Medical College)

  • Jeffrey S. Rush

    (University of Kentucky College of Medicine)

  • Charles J. Waechter

    (University of Kentucky College of Medicine)

  • Anant K. Menon

    (Weill Cornell Medical College)

Abstract

Arising from: J. Helenius et al. Nature 415, 447–450 (2002)10.1038/415447a ; Helenius et al. reply Protein N-glycosylation requires flipping of the glycolipid Man5GlcNAc2-diphosphate dolichol (Man5GlcNAc2-PP-Dol) across the endoplasmic reticulum (ER)1,2,3. Helenius et al.4 report genetic evidence suggesting that Rft1, an essential ER membrane protein in yeast, is required directly to translocate Man5GlcNAc2-PP-Dol. We now show that a specific ER protein(s), but not Rft1, is required to flip Man5GlcNAc2-PP-Dol in reconstituted vesicles. Rft1 may have a critical accessory role in translocating Man5GlcNAc2-PP-Dol in vivo, but the Man5GlcNAc2-PP-Dol flippase itself remains to be identified.

Suggested Citation

  • Christian G. Frank & Sumana Sanyal & Jeffrey S. Rush & Charles J. Waechter & Anant K. Menon, 2008. "Does Rft1 flip an N-glycan lipid precursor?," Nature, Nature, vol. 454(7204), pages 3-4, July.
    • Handle: RePEc:nat:nature:v:454:y:2008:i:7204:d:10.1038_nature07165
    DOI: 10.1038/nature07165
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