IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v453y2008i7193d10.1038_nature06893.html
   My bibliography  Save this article

3.88 Å structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy

Author

Listed:
  • Xuekui Yu

    (The University of Texas Medical School at Houston, Houston, Texas 77030, USA
    Immunology & Molecular Genetics and The California NanoSystems Institute, University of California at Los Angeles, Los Angeles, California 90095-1594, USA)

  • Lei Jin

    (The University of Texas Medical School at Houston, Houston, Texas 77030, USA
    Immunology & Molecular Genetics and The California NanoSystems Institute, University of California at Los Angeles, Los Angeles, California 90095-1594, USA)

  • Z. Hong Zhou

    (The University of Texas Medical School at Houston, Houston, Texas 77030, USA
    Immunology & Molecular Genetics and The California NanoSystems Institute, University of California at Los Angeles, Los Angeles, California 90095-1594, USA)

Abstract

Cytoplasmic polyhedrosis virus Cytoplasmic polyhedrosis virus (CPV) is a member of a large family of double-stranded RNA viruses, but it is unique in having a single shell capsid yet being fully capable of cell entry and mRNA transcription. The structure of this virus has now been determined by single-particle cryo-electron microscopy (cryoEM) to a resolution of 3.88 Å. The technique allows the polypeptide backbone to be traced without the need to make a crystal. The high-resolution structure shows how conformational switching is exploited to make railroad-like 'sliding' tracks for RNA packing and transcription and reveals an mRNA releasing hole coupled with distinctive capping machinery. With this and several other recent publications, cryo-electron microscopy underlines its credentials as a system capable of atomic-resolution in structural studies.

Suggested Citation

  • Xuekui Yu & Lei Jin & Z. Hong Zhou, 2008. "3.88 Å structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy," Nature, Nature, vol. 453(7193), pages 415-419, May.
    • Handle: RePEc:nat:nature:v:453:y:2008:i:7193:d:10.1038_nature06893
    DOI: 10.1038/nature06893
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature06893
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature06893?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:453:y:2008:i:7193:d:10.1038_nature06893. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.