IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v446y2007i7134d10.1038_nature05625.html
   My bibliography  Save this article

Peptide bond formation destabilizes Shine–Dalgarno interaction on the ribosome

Author

Listed:
  • Sotaro Uemura

    (Department of Physics,)

  • Magdalena Dorywalska

    (Stanford University, Stanford, California 94305, USA)

  • Tae-Hee Lee

    (Department of Physics,)

  • Harold D. Kim

    (Department of Physics,)

  • Joseph D. Puglisi

    (Stanford University, Stanford, California 94305, USA)

  • Steven Chu

    (Department of Physics,
    Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    Molecular and Cellular Biology, University of California, Berkeley, California 94720, USA)

Abstract

How the Shine–Dalgarno (SD) sequence affects binding of the ribosome to mRNA is examined by measuring the force required to tear the ribosome from an mRNA with or without the SD sequence. This finds that the SD sequence stabilizes the ribosome–mRNA interaction before the first peptide bond is formed, but that once this occurs, the SD sequence can no longer stabilize it.

Suggested Citation

  • Sotaro Uemura & Magdalena Dorywalska & Tae-Hee Lee & Harold D. Kim & Joseph D. Puglisi & Steven Chu, 2007. "Peptide bond formation destabilizes Shine–Dalgarno interaction on the ribosome," Nature, Nature, vol. 446(7134), pages 454-457, March.
    • Handle: RePEc:nat:nature:v:446:y:2007:i:7134:d:10.1038_nature05625
    DOI: 10.1038/nature05625
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature05625
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature05625?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:446:y:2007:i:7134:d:10.1038_nature05625. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.