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Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I

Author

Listed:
  • Christian G. Riedel

    (Research Institute of Molecular Pathology (IMP))

  • Vittorio L. Katis

    (Research Institute of Molecular Pathology (IMP)
    University of Oxford)

  • Yuki Katou

    (Tokyo Institute of Technology)

  • Saori Mori

    (Tokyo Institute of Technology)

  • Takehiko Itoh

    (Mitsubishi Research Institute Inc.)

  • Wolfgang Helmhart

    (Research Institute of Molecular Pathology (IMP)
    University of Oxford)

  • Marta Gálová

    (Research Institute of Molecular Pathology (IMP))

  • Mark Petronczki

    (Research Institute of Molecular Pathology (IMP))

  • Juraj Gregan

    (Research Institute of Molecular Pathology (IMP))

  • Bulent Cetin

    (Research Institute of Molecular Pathology (IMP)
    University of Oxford)

  • Ingrid Mudrak

    (Medical University of Vienna)

  • Egon Ogris

    (Medical University of Vienna)

  • Karl Mechtler

    (Research Institute of Molecular Pathology (IMP))

  • Laurence Pelletier

    (Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG))

  • Frank Buchholz

    (Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG))

  • Katsuhiko Shirahige

    (Tokyo Institute of Technology)

  • Kim Nasmyth

    (Research Institute of Molecular Pathology (IMP)
    University of Oxford)

Abstract

Segregation of homologous maternal and paternal centromeres to opposite poles during meiosis I depends on post-replicative crossing over between homologous non-sister chromatids, which creates chiasmata and therefore bivalent chromosomes. Destruction of sister chromatid cohesion along chromosome arms due to proteolytic cleavage of cohesin's Rec8 subunit by separase resolves chiasmata and thereby triggers the first meiotic division. This produces univalent chromosomes, the chromatids of which are held together by centromeric cohesin that has been protected from separase by shugoshin (Sgo1/MEI-S332) proteins. Here we show in both fission and budding yeast that Sgo1 recruits to centromeres a specific form of protein phosphatase 2A (PP2A). Its inactivation causes loss of centromeric cohesin at anaphase I and random segregation of sister centromeres at the second meiotic division. Artificial recruitment of PP2A to chromosome arms prevents Rec8 phosphorylation and hinders resolution of chiasmata. Our data are consistent with the notion that efficient cleavage of Rec8 requires phosphorylation of cohesin and that this is blocked by PP2A at meiosis I centromeres.

Suggested Citation

  • Christian G. Riedel & Vittorio L. Katis & Yuki Katou & Saori Mori & Takehiko Itoh & Wolfgang Helmhart & Marta Gálová & Mark Petronczki & Juraj Gregan & Bulent Cetin & Ingrid Mudrak & Egon Ogris & Karl, 2006. "Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I," Nature, Nature, vol. 441(7089), pages 53-61, May.
    • Handle: RePEc:nat:nature:v:441:y:2006:i:7089:d:10.1038_nature04664
    DOI: 10.1038/nature04664
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    Cited by:

    1. Bo Pan & Melania Bruno & Todd S. Macfarlan & Takashi Akera, 2025. "Meiosis-specific distal cohesion site decoupled from the kinetochore," Nature Communications, Nature, vol. 16(1), pages 1-14, December.

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