IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v438y2005i7068d10.1038_nature04151.html
   My bibliography  Save this article

The APC/C and CBP/p300 cooperate to regulate transcription and cell-cycle progression

Author

Listed:
  • Andrew S. Turnell

    (The Medical School, The University of Birmingham)

  • Grant S. Stewart

    (The Medical School, The University of Birmingham
    Baylor College of Medicine
    Baylor College of Medicine)

  • Roger J. A. Grand

    (The Medical School, The University of Birmingham)

  • Susan M. Rookes

    (The Medical School, The University of Birmingham)

  • Ashley Martin

    (The Medical School, The University of Birmingham)

  • Hiroyuki Yamano

    (The Chart)

  • Stephen J. Elledge

    (Baylor College of Medicine
    Baylor College of Medicine
    Harvard University Medical School)

  • Phillip H. Gallimore

    (The Medical School, The University of Birmingham)

Abstract

The anaphase-promoting complex/cyclosome (APC/C) is a multicomponent E3 ubiquitin ligase that, by targeting protein substrates for 26S proteasome-mediated degradation through ubiquitination, coordinates the temporal progression of eukaryotic cells through mitosis and the subsequent G1 phase of the cell cycle1,2,3,4. Other functions of the APC/C are, however, less well defined. Here we show that two APC/C components, APC5 and APC7, interact directly with the coactivators CBP and p300 through protein–protein interaction domains that are evolutionarily conserved in adenovirus E1A5,6,7,8. This interaction stimulates intrinsic CBP/p300 acetyltransferase activity and potentiates CBP/p300-dependent transcription. We also show that APC5 and APC7 suppress E1A-mediated transformation in a CBP/p300-dependent manner, indicating that these components of the APC/C may be targeted during cellular transformation. Furthermore, we establish that CBP is required in APC/C function; specifically, gene ablation of CBP by RNA-mediated interference markedly reduces the E3 ubiquitin ligase activity of the APC/C and the progression of cells through mitosis. Taken together, our results define discrete roles for the APC/C–CBP/p300 complexes in growth regulation.

Suggested Citation

  • Andrew S. Turnell & Grant S. Stewart & Roger J. A. Grand & Susan M. Rookes & Ashley Martin & Hiroyuki Yamano & Stephen J. Elledge & Phillip H. Gallimore, 2005. "The APC/C and CBP/p300 cooperate to regulate transcription and cell-cycle progression," Nature, Nature, vol. 438(7068), pages 690-695, December.
    • Handle: RePEc:nat:nature:v:438:y:2005:i:7068:d:10.1038_nature04151
    DOI: 10.1038/nature04151
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature04151
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature04151?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:438:y:2005:i:7068:d:10.1038_nature04151. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.