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Structure of the E. coli protein-conducting channel bound to a translating ribosome

Author

Listed:
  • Kakoli Mitra

    (Health Research Inc. at the Wadsworth Center, Empire State Plaza)

  • Christiane Schaffitzel

    (Eidgenössische Technische Hochschule Hönggerberg)

  • Tanvir Shaikh

    (Wadsworth Center, Empire State Plaza)

  • Florence Tama

    (The Scripps Research Institute)

  • Simon Jenni

    (Eidgenössische Technische Hochschule Hönggerberg)

  • Charles L. Brooks

    (The Scripps Research Institute)

  • Nenad Ban

    (Eidgenössische Technische Hochschule Hönggerberg)

  • Joachim Frank

    (Health Research Inc. at the Wadsworth Center, Empire State Plaza
    Wadsworth Center, Empire State Plaza
    State University of New York at Albany, Empire State Plaza)

Abstract

Secreted and membrane proteins are translocated across or into cell membranes through a protein-conducting channel (PCC). Here we present a cryo-electron microscopy reconstruction of the Escherichia coli PCC, SecYEG, complexed with the ribosome and a nascent chain containing a signal anchor. This reconstruction shows a messenger RNA, three transfer RNAs, the nascent chain, and detailed features of both a translocating PCC and a second, non-translocating PCC bound to mRNA hairpins. The translocating PCC forms connections with ribosomal RNA hairpins on two sides and ribosomal proteins at the back, leaving a frontal opening. Normal mode-based flexible fitting of the archaeal SecYEβ structure into the PCC electron microscopy densities favours a front-to-front arrangement of two SecYEG complexes in the PCC, and supports channel formation by the opening of two linked SecY halves during polypeptide translocation. On the basis of our observation in the translocating PCC of two segregated pores with different degrees of access to bulk lipid, we propose a model for co-translational protein translocation.

Suggested Citation

  • Kakoli Mitra & Christiane Schaffitzel & Tanvir Shaikh & Florence Tama & Simon Jenni & Charles L. Brooks & Nenad Ban & Joachim Frank, 2005. "Structure of the E. coli protein-conducting channel bound to a translating ribosome," Nature, Nature, vol. 438(7066), pages 318-324, November.
    • Handle: RePEc:nat:nature:v:438:y:2005:i:7066:d:10.1038_nature04133
    DOI: 10.1038/nature04133
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