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Phosphatidylserine-dependent engulfment by macrophages of nuclei from erythroid precursor cells

Author

Listed:
  • Hideyuki Yoshida

    (Osaka University)

  • Kohki Kawane

    (Osaka University
    Osaka University
    Japan Science and Technology Corporation)

  • Masato Koike

    (Osaka University)

  • Yoshimi Mori

    (Sakura Motion Picture Co. Ltd)

  • Yasuo Uchiyama

    (Osaka University)

  • Shigekazu Nagata

    (Osaka University
    Osaka University
    Japan Science and Technology Corporation)

Abstract

Definitive erythropoiesis usually occurs in the bone marrow or fetal liver, where erythroblasts are associated with a central macrophage in anatomical units called ‘blood islands’1,2. Late in erythropoiesis, nuclei are expelled from the erythroid precursor cells and engulfed by the macrophages in the blood island2,3. Here we show that the nuclei are engulfed by macrophages only after they are disconnected from reticulocytes, and that phosphatidylserine, which is often used as an ‘eat me’ signal for apoptotic cells, is also used for the engulfment of nuclei expelled from erythroblasts. We investigated the mechanism behind the enucleation and engulfment processes by isolating late-stage erythroblasts from the spleens of phlebotomized mice. When these erythroblasts were cultured, the nuclei protruded spontaneously from the erythroblasts. A weak physical force could disconnect the nuclei from the reticulocytes. The released nuclei contained an undetectable level of ATP, and quickly exposed phosphatidylserine on their surface. Fetal liver macrophages efficiently engulfed the nuclei; masking the phosphatidylserine on the nuclei with the dominant-negative form of milk-fat-globule EGF8 (MFG-E8) prevented this engulfment.

Suggested Citation

  • Hideyuki Yoshida & Kohki Kawane & Masato Koike & Yoshimi Mori & Yasuo Uchiyama & Shigekazu Nagata, 2005. "Phosphatidylserine-dependent engulfment by macrophages of nuclei from erythroid precursor cells," Nature, Nature, vol. 437(7059), pages 754-758, September.
  • Handle: RePEc:nat:nature:v:437:y:2005:i:7059:d:10.1038_nature03964
    DOI: 10.1038/nature03964
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