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A non-haem iron centre in the transcription factor NorR senses nitric oxide

Author

Listed:
  • Benoît D'Autréaux

    (John Innes Centre, Colney)

  • Nicholas P. Tucker

    (John Innes Centre, Colney)

  • Ray Dixon

    (John Innes Centre, Colney)

  • Stephen Spiro

    (Georgia Institute of Technology)

Abstract

Nitric oxide (NO), synthesized in eukaryotes by the NO synthases, has multiple roles in signalling pathways and in protection against pathogens1,2. Pathogenic microorganisms have apparently evolved defence mechanisms that counteract the effects of NO and related reactive nitrogen species. Regulatory proteins that sense NO mediate the primary response to NO and nitrosative stress3,4,5,6,7,8,9. The only regulatory protein in enteric bacteria known to serve exclusively as an NO-responsive transcription factor is the enhancer binding protein NorR (refs 9, 10–11). In Escherichia coli, NorR activates the transcription of the norVW genes encoding a flavorubredoxin (FlRd) and an associated flavoprotein, respectively, which together have NADH-dependent NO reductase activity10,12,13,14. The NO-responsive activity of NorR raises important questions concerning the mechanism of NO sensing. Here we show that the regulatory domain of NorR contains a mononuclear non-haem iron centre, which reversibly binds NO. Binding of NO stimulates the ATPase activity of NorR, enabling the activation of transcription by RNA polymerase. The mechanism of NorR reveals an unprecedented biological role for a non-haem mononitrosyl–iron complex in NO sensing.

Suggested Citation

  • Benoît D'Autréaux & Nicholas P. Tucker & Ray Dixon & Stephen Spiro, 2005. "A non-haem iron centre in the transcription factor NorR senses nitric oxide," Nature, Nature, vol. 437(7059), pages 769-772, September.
    • Handle: RePEc:nat:nature:v:437:y:2005:i:7059:d:10.1038_nature03953
    DOI: 10.1038/nature03953
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