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Hoogsteen base-pairing in DNA replication?

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  • Jimin Wang

    (Center for Structural Biology, Yale University)

Abstract

Arising from: D. T. Nair, R. E. Johnson, S. Prakash, L. Prakash & A. K. Aggarwal Nature 430, 377–380 (2004); Nair et al. reply . Human polymerase-ι belongs to the error-prone Y family of polymerases, which frequently incorporate incorrect nucleotides during DNA replication but can efficiently bypass DNA lesions1,2. On the basis of X-ray diffraction data, Nair et al. propose that Hoogsteen base-pairing is adopted by DNA during its replication by this enzyme3. Here I re-examine their X-ray data and find that the electron density is very weak for a Hoogsteen base pair formed between a template adenine deoxyribonucleotide in the syn conformation and a deoxythymidine 5′-triphosphate (dTTP), and that the fit is better for a normal Watson–Crick base pair. As a guanine–cytosine (G–C) base pair has no potential to form a Hoogsteen base pair at physiological pH, Hoogsteen base-pairing is unlikely to be used in replication by this polymerase.

Suggested Citation

  • Jimin Wang, 2005. "Hoogsteen base-pairing in DNA replication?," Nature, Nature, vol. 437(7057), pages 6-7, September.
    • Handle: RePEc:nat:nature:v:437:y:2005:i:7057:d:10.1038_nature04199
    DOI: 10.1038/nature04199
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