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The transcription factor Ifh1 is a key regulator of yeast ribosomal protein genes

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  • Joseph T. Wade

    (Harvard Medical School)

  • Daniel B. Hall

    (Harvard Medical School)

  • Kevin Struhl

    (Harvard Medical School)

Abstract

Ribosomal protein (RP) genes in eukaryotes are coordinately regulated in response to growth stimuli and environmental stress, thereby permitting cells to adjust ribosome number and overall protein synthetic capacity to physiological conditions1,2,3,4,5. Approximately 50% of RNA polymerase II transcription is devoted to RP genes5. The transcriptional regulator Rap1 binds most yeast RP promoters6, and Rap1 sites are important for coordinate regulation of RP genes7,8,9,10. However, Rap1 is not the specific regulator that controls RP transcription because it also functions as a repressor, and many Rap1-activated promoters are not coordinately regulated with RP promoters11,12. Here we show that the transcription factors Fhl1 and Ifh1 associate almost exclusively with RP promoters; association depends on Rap1 and (to a lesser extent) a DNA element at many RP promoters. Ifh1 is recruited to promoters via the forkhead-associated (FHA) domain of Fhl1; the level of Ifh1 associated with RP promoters determines the level of transcription; and environmental stress causes a marked reduction in the association of Ifh1, but not Fhl1 or Rap1. Thus, Ifh1 association with promoters is the key regulatory step for coordinate expression of RP genes.

Suggested Citation

  • Joseph T. Wade & Daniel B. Hall & Kevin Struhl, 2004. "The transcription factor Ifh1 is a key regulator of yeast ribosomal protein genes," Nature, Nature, vol. 432(7020), pages 1054-1058, December.
    • Handle: RePEc:nat:nature:v:432:y:2004:i:7020:d:10.1038_nature03175
    DOI: 10.1038/nature03175
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    Cited by:

    1. Yuanyuan Xiao & Yee H Yang & Todd A Burckin & Lily Shiue & Grant A Hartzog & Mark R Segal, 2005. "Analysis of a Splice Array Experiment Elucidates Roles of Chromatin Elongation Factor Spt4–5 in Splicing," PLOS Computational Biology, Public Library of Science, vol. 1(4), pages 1-1, September.

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