IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v430y2004i7002d10.1038_nature02767.html
   My bibliography  Save this article

Cell cycle regulation of central spindle assembly

Author

Listed:
  • Masanori Mishima

    (Research Institute of Molecular Pathology)

  • Visnja Pavicic

    (Research Institute of Molecular Pathology)

  • Ulrike Grüneberg

    (Max-Planck-Institute für Biochemie)

  • Erich A. Nigg

    (Max-Planck-Institute für Biochemie)

  • Michael Glotzer

    (Research Institute of Molecular Pathology)

Abstract

The bipolar mitotic spindle is responsible for segregating sister chromatids at anaphase. Microtubule motor proteins generate spindle bipolarity and enable the spindle to perform mechanical work1. A major change in spindle architecture occurs at anaphase onset when central spindle assembly begins. This structure regulates the initiation of cytokinesis and is essential for its completion2. Central spindle assembly requires the centralspindlin complex composed of the Caenorhabditis elegans ZEN-4 (mammalian orthologue MKLP1) kinesin-like protein and the Rho family GAP CYK-4 (MgcRacGAP). Here we describe a regulatory mechanism that controls the timing of central spindle assembly. The mitotic kinase Cdk1/cyclin B phosphorylates the motor domain of ZEN-4 on a conserved site within a basic amino-terminal extension characteristic of the MKLP1 subfamily. Phosphorylation by Cdk1 diminishes the motor activity of ZEN-4 by reducing its affinity for microtubules. Preventing Cdk1 phosphorylation of ZEN-4/MKLP1 causes enhanced metaphase spindle localization and defects in chromosome segregation. Thus, phosphoregulation of the motor domain of MKLP1 kinesin ensures that central spindle assembly occurs at the appropriate time in the cell cycle and maintains genomic stability.

Suggested Citation

  • Masanori Mishima & Visnja Pavicic & Ulrike Grüneberg & Erich A. Nigg & Michael Glotzer, 2004. "Cell cycle regulation of central spindle assembly," Nature, Nature, vol. 430(7002), pages 908-913, August.
    • Handle: RePEc:nat:nature:v:430:y:2004:i:7002:d:10.1038_nature02767
    DOI: 10.1038/nature02767
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature02767
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature02767?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:430:y:2004:i:7002:d:10.1038_nature02767. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.