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Is rhodopsin dimeric in native retinal rods?

Author

Listed:
  • Dimitrios Fotiadis

    (M.E. Müller Institute for Microscopy, Biozentrum, University of Basel)

  • Yan Liang

    (University of Washington)

  • Slawomir Filipek

    (International Institute of Molecular and Cell Biology, University of Warsaw)

  • David A. Saperstein

    (University of Washington)

  • Andreas Engel

    (M.E. Müller Institute for Microscopy, Biozentrum, University of Basel)

  • Krzysztof Palczewski

    (University of Washington
    University of Washington
    University of Washington)

Abstract

Individual biological molecules can be imaged under physiological conditions by atomic force microscopy. Our results from AFM, supported by electron microscopy, revealed distinct rows of rhodopsin dimers and paracrystalline arrays in native murine disc membranes1,2. This supramolecular arrangement was also found for the light-activated form, opsin1. We counted 30,000–55,000 rhodopsin molecules per square micrometre, a packing density comparable to that measured by optical methods in amphibian discs3. From the lattice vectors describing the paracrystalline arrays, a maximum possible packing density of 62,900 rhodopsin molecules per square micrometre was calculated. The rhodopsin dimers seen in AFM topographs2 have cytosolic protrusions separated by 3.8 nm, providing an ideal docking platform for arrestin, which has two binding grooves that are separated by 3.8 nm as well1.

Suggested Citation

  • Dimitrios Fotiadis & Yan Liang & Slawomir Filipek & David A. Saperstein & Andreas Engel & Krzysztof Palczewski, 2003. "Is rhodopsin dimeric in native retinal rods?," Nature, Nature, vol. 426(6962), pages 31-31, November.
    • Handle: RePEc:nat:nature:v:426:y:2003:i:6962:d:10.1038_426031a
    DOI: 10.1038/426031a
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