IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v415y2002i6873d10.1038_415810a.html
   My bibliography  Save this article

The non-coding Air RNA is required for silencing autosomal imprinted genes

Author

Listed:
  • Frank Sleutels

    (The Netherlands Cancer Institute)

  • Ronald Zwart

    (The Netherlands Cancer Institute)

  • Denise P. Barlow

    (ÖAW Institute of Molecular Biology)

Abstract

In genomic imprinting, one of the two parental alleles of an autosomal gene is silenced epigenetically by a cis-acting mechanism1,2. A bidirectional silencer for a 400-kilobase region that contains three imprinted, maternally expressed protein-coding genes (Igf2r/Slc22a2/Slc22a3) has been shown by targeted deletion to be located in a sequence of 3.7 kilobases3,4,5, which also contains the promoter for the imprinted, paternally expressed non-coding Air RNA6. Expression of Air is correlated with repression of all three genes on the paternal allele5; however, Air RNA overlaps just one of these genes in an antisense orientation6. Here we show, by inserting a polyadenylation signal that truncates 96% of the RNA transcript, that Air RNA is required for silencing. The truncated Air allele maintains imprinted expression and methylation of the Air promoter, but shows complete loss of silencing of the Igf2r/Slc22a2/Slc22a3 gene cluster on the paternal chromosome. Our results indicate that non-coding RNAs have an active role in genomic imprinting.

Suggested Citation

  • Frank Sleutels & Ronald Zwart & Denise P. Barlow, 2002. "The non-coding Air RNA is required for silencing autosomal imprinted genes," Nature, Nature, vol. 415(6873), pages 810-813, February.
    • Handle: RePEc:nat:nature:v:415:y:2002:i:6873:d:10.1038_415810a
    DOI: 10.1038/415810a
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/415810a
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/415810a?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Lali Mwamaka Sharifu & Li jia qi & Feng Ling & Wang Shao shuai & Joyce Wanjiru Maingi & Paul Kibaba & Alfred Martin Omondi, 2020. "Imprinted Gene Regulation in the Placenta and Fetal Development," Biomedical Journal of Scientific & Technical Research, Biomedical Research Network+, LLC, vol. 28(3), pages 21560-21567, June.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:415:y:2002:i:6873:d:10.1038_415810a. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.