IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v409y2001i6819d10.1038_35054095.html
   My bibliography  Save this article

Genomic binding sites of the yeast cell-cycle transcription factors SBF and MBF

Author

Listed:
  • Vishwanath R. Iyer

    (Stanford University Medical Center
    University of Texas at Austin)

  • Christine E. Horak

    (Cellular and Developmental Biology, Yale University
    University of Texas at Austin)

  • Charles S. Scafe

    (Stanford University Medical Center
    Applied Biosystems)

  • David Botstein

    (Stanford University Medical Center)

  • Michael Snyder

    (Cellular and Developmental Biology, Yale University)

  • Patrick O. Brown

    (Stanford University Medical Center
    Howard Hughes Medical Institute, Stanford University Medical Center)

Abstract

Proteins interact with genomic DNA to bring the genome to life; and these interactions also define many functional features of the genome. SBF and MBF are sequence-specific transcription factors that activate gene expression during the G1/S transition of the cell cycle in yeast1,2. SBF is a heterodimer of Swi4 and Swi6, and MBF is a heterodimer of Mbp1 and Swi6 (refs 1, 3). The related Swi4 and Mbp1 proteins are the DNA-binding components of the respective factors, and Swi6 may have a regulatory function4,5. A small number of SBF and MBF target genes have been identified3,6,7,8,9,10. Here we define the genomic binding sites of the SBF and MBF transcription factors in vivo, by using DNA microarrays. In addition to the previously characterized targets, we have identified about 200 new putative targets. Our results support the hypothesis that SBF activated genes are predominantly involved in budding, and in membrane and cell-wall biosynthesis, whereas DNA replication and repair are the dominant functions among MBF activated genes6,11. The functional specialization of these factors may provide a mechanism for independent regulation of distinct molecular processes that normally occur in synchrony during the mitotic cell cycle.

Suggested Citation

  • Vishwanath R. Iyer & Christine E. Horak & Charles S. Scafe & David Botstein & Michael Snyder & Patrick O. Brown, 2001. "Genomic binding sites of the yeast cell-cycle transcription factors SBF and MBF," Nature, Nature, vol. 409(6819), pages 533-538, January.
    • Handle: RePEc:nat:nature:v:409:y:2001:i:6819:d:10.1038_35054095
    DOI: 10.1038/35054095
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/35054095
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/35054095?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Zhengdong D Zhang & Joel Rozowsky & Michael Snyder & Joseph Chang & Mark Gerstein, 2008. "Modeling ChIP Sequencing In Silico with Applications," PLOS Computational Biology, Public Library of Science, vol. 4(8), pages 1-10, August.
    2. Cheemeng Tan & Robert Phillip Smith & Ming-Chi Tsai & Russell Schwartz & Lingchong You, 2014. "Phenotypic Signatures Arising from Unbalanced Bacterial Growth," PLOS Computational Biology, Public Library of Science, vol. 10(8), pages 1-10, August.
    3. G. Saharidis & I. Androulakis & M. Ierapetritou, 2011. "Model building using bi-level optimization," Journal of Global Optimization, Springer, vol. 49(1), pages 49-67, January.
    4. John E Reid & Lorenz Wernisch, 2014. "STEME: A Robust, Accurate Motif Finder for Large Data Sets," PLOS ONE, Public Library of Science, vol. 9(3), pages 1-11, March.
    5. Kyoung-Jae Won & Saurabh Agarwal & Li Shen & Robert Shoemaker & Bing Ren & Wei Wang, 2009. "An Integrated Approach to Identifying Cis-Regulatory Modules in the Human Genome," PLOS ONE, Public Library of Science, vol. 4(5), pages 1-8, May.
    6. Emily N Manderson & Mohan Malleshaiah & Stephen W Michnick, 2008. "A Novel Genetic Screen Implicates Elm1 in the Inactivation of the Yeast Transcription Factor SBF," PLOS ONE, Public Library of Science, vol. 3(1), pages 1-9, January.
    7. Xinyi Liu & Bin Liu & Zhimin Huang & Ting Shi & Yingyi Chen & Jian Zhang, 2012. "SPPS: A Sequence-Based Method for Predicting Probability of Protein-Protein Interaction Partners," PLOS ONE, Public Library of Science, vol. 7(1), pages 1-6, January.
    8. Eilon Sharon & Shai Lubliner & Eran Segal, 2008. "A Feature-Based Approach to Modeling Protein–DNA Interactions," PLOS Computational Biology, Public Library of Science, vol. 4(8), pages 1-17, August.
    9. Xun Lan & Christopher Adams & Mark Landers & Miroslav Dudas & Daniel Krissinger & George Marnellos & Russell Bonneville & Maoxiong Xu & Junbai Wang & Tim H-M Huang & Gavin Meredith & Victor X Jin, 2011. "High Resolution Detection and Analysis of CpG Dinucleotides Methylation Using MBD-Seq Technology," PLOS ONE, Public Library of Science, vol. 6(7), pages 1-11, July.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:409:y:2001:i:6819:d:10.1038_35054095. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.