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Chromosomal landscape of nucleosome-dependent gene expression and silencing in yeast

Author

Listed:
  • John J. Wyrick

    (Whitehead Institute for Biomedical Research, Nine Cambridge Center
    Massachusetts Institute of Technology)

  • Frank C. P. Holstege

    (Whitehead Institute for Biomedical Research, Nine Cambridge Center)

  • Ezra G. Jennings

    (Whitehead Institute for Biomedical Research, Nine Cambridge Center
    Massachusetts Institute of Technology)

  • Helen C. Causton

    (Whitehead Institute for Biomedical Research, Nine Cambridge Center)

  • David Shore

    (University of Geneva)

  • Michael Grunstein

    (UCLA School of Medicine and Molecular Biology Institute, University of California)

  • Eric S. Lander

    (Whitehead Institute for Biomedical Research, Nine Cambridge Center
    Massachusetts Institute of Technology)

  • Richard A. Young

    (Massachusetts Institute of Technology)

Abstract

Eukaryotic genomes are packaged into nucleosomes, which are thought to repress gene expression generally1,2,3. Repression is particularly evident at yeast telomeres, where genes within the telomeric heterochromatin appear to be silenced by the histone-binding silent information regulator (SIR) complex (Sir2, Sir3, Sir4) and Rap1 (refs 4,5,6,7,8,9,10). Here, to investigate how nucleosomes and silencing factors influence global gene expression, we use high-density arrays to study the effects of depleting nucleosomal histones and silencing factors in yeast. Reducing nucleosome content by depleting histone H4 caused increased expression of 15% of genes and reduced expression of 10% of genes, but it had little effect on expression of the majority (75%) of yeast genes. Telomere-proximal genes were found to be de-repressed over regions extending 20 kilobases from the telomeres, well beyond the extent of Sir protein binding11,12 and the effects of loss of Sir function. These results indicate that histones make Sir-independent contributions to telomeric silencing, and that the role of histones located elsewhere in chromosomes is gene specific rather than generally repressive.

Suggested Citation

  • John J. Wyrick & Frank C. P. Holstege & Ezra G. Jennings & Helen C. Causton & David Shore & Michael Grunstein & Eric S. Lander & Richard A. Young, 1999. "Chromosomal landscape of nucleosome-dependent gene expression and silencing in yeast," Nature, Nature, vol. 402(6760), pages 418-421, November.
    • Handle: RePEc:nat:nature:v:402:y:1999:i:6760:d:10.1038_46567
    DOI: 10.1038/46567
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