IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v391y1998i6663d10.1038_34448.html
   My bibliography  Save this article

Interaction of a G-protein β-subunit with a conserved sequence in Ste20/PAK family protein kinases

Author

Listed:
  • Thomas Leeuw

    (Eukaryotic Genetics Group, National Research Council of Canada)

  • Cunle Wu

    (Eukaryotic Genetics Group, National Research Council of Canada)

  • Joseph D. Schrag

    (Macromolecular Structure Group, Biotechnology Research Institute, National Research Council of Canada)

  • Malcolm Whiteway

    (Eukaryotic Genetics Group, National Research Council of Canada
    McGill University)

  • David Y. Thomas

    (Eukaryotic Genetics Group, National Research Council of Canada
    McGill University
    McGill University)

  • Ekkehard Leberer

    (Eukaryotic Genetics Group, National Research Council of Canada
    McGill University)

Abstract

Serine/threonine protein kinases of the Ste20/PAK family have been implicated in the signalling from heterotrimeric G proteins to mitogen-activated protein (MAP) kinase cascades1,2. In the yeast Saccharomyces cerevisiae, Ste20 is involved in transmitting the mating-pheromone signal from the βγ-subunits (encoded by the STE4 and STE18 genes, respectively) of a heterotrimeric G protein to a downstream MAP kinase cascade1. We have identified a binding site for the G-protein β-subunit (Gβ) in the non-catalytic carboxy-terminal regions of Ste20 and its mammalian homologues, the p21-activated protein kinases (PAKs). Association of Gβ with this site in Ste20 was regulated by binding of pheromone to the receptor. Mutations in Gβ and Ste20 that prevented this association blocked activation of the MAP kinase cascade. Considering the high degree of structural and functional conservation of Ste20/PAK family members and G-protein subunits, our results provide a possible model for a role of these kinases in Gβγ-mediated signal transduction in organisms ranging from yeast to mammals.

Suggested Citation

  • Thomas Leeuw & Cunle Wu & Joseph D. Schrag & Malcolm Whiteway & David Y. Thomas & Ekkehard Leberer, 1998. "Interaction of a G-protein β-subunit with a conserved sequence in Ste20/PAK family protein kinases," Nature, Nature, vol. 391(6663), pages 191-195, January.
  • Handle: RePEc:nat:nature:v:391:y:1998:i:6663:d:10.1038_34448
    DOI: 10.1038/34448
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/34448
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.

    File URL: https://libkey.io/10.1038/34448?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:391:y:1998:i:6663:d:10.1038_34448. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.