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Calcium turns turquoise into gold

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  • Tullio Pozzan

    (CNR Centre of Biomembranes, University of Padova)

Abstract

The concentration of calcium in living cells can be measured using differently coloured green fluorescent protein (GFP) mutants. Two groups have independently linked blue-shifted GFP mutants to longer-wavelength GFP mutants through connecting sequences to which Ca2+-calmodulin or Ca2+ alone can bind. Fluorescence energy resonance transfer occurs between the two mutants, but it is very sensitive to the distance and orientation of the fluorophores, and so can detect Ca2+ binding.

Suggested Citation

  • Tullio Pozzan, 1997. "Calcium turns turquoise into gold," Nature, Nature, vol. 388(6645), pages 834-835, August.
    • Handle: RePEc:nat:nature:v:388:y:1997:i:6645:d:10.1038_42151
    DOI: 10.1038/42151
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