IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v385y1997i6619d10.1038_385826a0.html
   My bibliography  Save this article

Hrs-2 is an ATPase implicated in calcium-regulated secretion

Author

Listed:
  • Andrew J. Bean

    (Stanford University School of Medicine)

  • Roland Seifert

    (Stanford University School of Medicine)

  • Yu A. Chen

    (Stanford University School of Medicine)

  • Rachel Sacks

    (Stanford University School of Medicine)

  • Richard H. Scheller

    (Stanford University School of Medicine)

Abstract

Associations between proteins present on neurotransmitter-containing vesicles and on the presynaptic membrane are thought to underlie docking and fusion of synaptic vesicles with the plasma membrane, which are obligate steps in regulated neurotransmission1–4. SNAP-25 resides on the plasma membrane and interacts with syntaxin (a plasma membrane t-SNARE) and VAMP (a vesicle v-SNARE)1–9 to form a core protein complex thought to be an intermediate in a biochemical pathway that is essential for vesicular transport. We have now characterized a protein, Hrs-2, that interacts with SNAP-25. The binding of Hrs-2 to SNAP-25 is inhibited by calcium in the physiological concentration range that supports synaptic transmission. Furthermore, Hrs-2 binds and hydrolyses nucleoside triphosphates with kinetics that suggest that ATP is the physiological substrate for this enzyme. Hrs-2 is expressed throughout the brain and is present in nerve terminals. Moreover, recombinant Hrs-2 inhibits calcium-triggered 3H-noradrenaline release from permeabilized PC 12 cells. Our results suggest a role for Hrs-2 in regulating secretory processes through calcium- and nucleotide-dependent modulation of vesicle-trafficking protein complexes.

Suggested Citation

  • Andrew J. Bean & Roland Seifert & Yu A. Chen & Rachel Sacks & Richard H. Scheller, 1997. "Hrs-2 is an ATPase implicated in calcium-regulated secretion," Nature, Nature, vol. 385(6619), pages 826-829, February.
    • Handle: RePEc:nat:nature:v:385:y:1997:i:6619:d:10.1038_385826a0
    DOI: 10.1038/385826a0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/385826a0
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/385826a0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:385:y:1997:i:6619:d:10.1038_385826a0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.