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Differentiation of primate primordial germ cell-like cells following transplantation into the adult gonadal niche

Author

Listed:
  • Enrique Sosa

    (University of California, Los Angeles)

  • Di Chen

    (University of California, Los Angeles)

  • Ernesto J. Rojas

    (University of California, Los Angeles)

  • Jon D. Hennebold

    (Oregon National Primate Research Center
    Oregon Health & Science University)

  • Karen A. Peters

    (University of Pittsburgh School of Medicine)

  • Zhuang Wu

    (University of Texas MD Anderson Cancer Center)

  • Truong N. Lam

    (University of Texas MD Anderson Cancer Center)

  • Jennifer M. Mitchell

    (University of Texas MD Anderson Cancer Center)

  • Meena Sukhwani

    (University of Pittsburgh School of Medicine)

  • Ramesh C. Tailor

    (University of Texas MD Anderson Cancer Center)

  • Marvin L. Meistrich

    (University of Texas MD Anderson Cancer Center)

  • Kyle E. Orwig

    (University of Pittsburgh School of Medicine)

  • Gunapala Shetty

    (University of Texas MD Anderson Cancer Center)

  • Amander T. Clark

    (University of California, Los Angeles)

Abstract

A major challenge in stem cell differentiation is the availability of bioassays to prove cell types generated in vitro are equivalent to cells in vivo. In the mouse, differentiation of primordial germ cell-like cells (PGCLCs) from pluripotent cells was validated by transplantation, leading to the generation of spermatogenesis and to the birth of offspring. Here we report the use of xenotransplantation (monkey to mouse) and homologous transplantation (monkey to monkey) to validate our in vitro protocol for differentiating male rhesus (r) macaque PGCLCs (rPGCLCs) from induced pluripotent stem cells (riPSCs). Specifically, transplantation of aggregates containing rPGCLCs into mouse and nonhuman primate testicles overcomes a major bottleneck in rPGCLC differentiation. These findings suggest that immature rPGCLCs once transplanted into an adult gonadal niche commit to differentiate towards late rPGCs that initiate epigenetic reprogramming but do not complete the conversion into ENO2-positive spermatogonia.

Suggested Citation

  • Enrique Sosa & Di Chen & Ernesto J. Rojas & Jon D. Hennebold & Karen A. Peters & Zhuang Wu & Truong N. Lam & Jennifer M. Mitchell & Meena Sukhwani & Ramesh C. Tailor & Marvin L. Meistrich & Kyle E. Or, 2018. "Differentiation of primate primordial germ cell-like cells following transplantation into the adult gonadal niche," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-07740-7
    DOI: 10.1038/s41467-018-07740-7
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    Cited by:

    1. Sivakamasundari Vijayakumar & Roberta Sala & Gugene Kang & Angela Chen & Michelle Ann Pablo & Abidemi Ismail Adebayo & Andrea Cipriano & Jonas L. Fowler & Danielle L. Gomes & Lay Teng Ang & Kyle M. Lo, 2023. "Monolayer platform to generate and purify primordial germ-like cells in vitro provides insights into human germline specification," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

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