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Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy

Author

Listed:
  • Youhua Chen

    (Zhejiang University, Hangzhou
    North University of China)

  • Wenjie Liu

    (Zhejiang University, Hangzhou)

  • Zhimin Zhang

    (Zhejiang University, Hangzhou)

  • Cheng Zheng

    (Zhejiang University, Hangzhou)

  • Yujia Huang

    (Zhejiang University, Hangzhou)

  • Ruizhi Cao

    (Zhejiang University, Hangzhou)

  • Dazhao Zhu

    (Zhejiang University, Hangzhou)

  • Liang Xu

    (Zhejiang University, Hangzhou)

  • Meng Zhang

    (Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology)

  • Yu-Hui Zhang

    (Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology)

  • Jiannan Fan

    (Zhejiang University)

  • Luhong Jin

    (Zhejiang University)

  • Yingke Xu

    (Zhejiang University)

  • Cuifang Kuang

    (Zhejiang University, Hangzhou
    Shanxi University)

  • Xu Liu

    (Zhejiang University, Hangzhou
    Shanxi University)

Abstract

Imaging and tracking of near-surface three-dimensional volumetric nanoscale dynamic processes of live cells remains a challenging problem. In this paper, we propose a multi-color live-cell near-surface-volume super-resolution microscopy method that combines total internal reflection fluorescence structured illumination microscopy with multi-angle evanescent light illumination. We demonstrate that our approach of multi-angle interference microscopy is perfectly adapted to studying subcellular dynamics of mitochondria and microtubule architectures during cell migration.

Suggested Citation

  • Youhua Chen & Wenjie Liu & Zhimin Zhang & Cheng Zheng & Yujia Huang & Ruizhi Cao & Dazhao Zhu & Liang Xu & Meng Zhang & Yu-Hui Zhang & Jiannan Fan & Luhong Jin & Yingke Xu & Cuifang Kuang & Xu Liu, 2018. "Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy," Nature Communications, Nature, vol. 9(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-07244-4
    DOI: 10.1038/s41467-018-07244-4
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