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Dynamics of individual molecular shuttles under mechanical force

Author

Listed:
  • Teresa Naranjo

    (Ciudad Universitaria de Cantoblanco)

  • Kateryna M. Lemishko

    (Nanobiotecnología (IMDEA-Nanociencia) Unidad Asociada al Centro Nacional de Biotecnología (CSIC))

  • Sara Lorenzo

    (Ciudad Universitaria de Cantoblanco)

  • Álvaro Somoza

    (Ciudad Universitaria de Cantoblanco
    Nanobiotecnología (IMDEA-Nanociencia) Unidad Asociada al Centro Nacional de Biotecnología (CSIC))

  • Felix Ritort

    (University of Barcelona
    Instituto de Sanidad Carlos III)

  • Emilio M. Pérez

    (Ciudad Universitaria de Cantoblanco)

  • Borja Ibarra

    (Ciudad Universitaria de Cantoblanco
    Nanobiotecnología (IMDEA-Nanociencia) Unidad Asociada al Centro Nacional de Biotecnología (CSIC))

Abstract

Molecular shuttles are the basis of some of the most advanced synthetic molecular machines. In these devices a macrocycle threaded onto a linear component shuttles between different portions of the thread in response to external stimuli. Here, we use optical tweezers to measure the mechanics and dynamics of individual molecular shuttles in aqueous conditions. Using DNA as a handle and as a single molecule reporter, we measure thousands of individual shuttling events and determine the force-dependent kinetic rates of the macrocycle motion and the main parameters governing the energy landscape of the system. Our findings could open avenues for the real-time characterization of synthetic devices at the single molecule level, and provide crucial information for designing molecular machinery able to operate under physiological conditions.

Suggested Citation

  • Teresa Naranjo & Kateryna M. Lemishko & Sara Lorenzo & Álvaro Somoza & Felix Ritort & Emilio M. Pérez & Borja Ibarra, 2018. "Dynamics of individual molecular shuttles under mechanical force," Nature Communications, Nature, vol. 9(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06905-8
    DOI: 10.1038/s41467-018-06905-8
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