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Early redox activities modulate Xenopus tail regeneration

Author

Listed:
  • Fernando Ferreira

    (University of California
    Universidade do Minho)

  • VijayKrishna Raghunathan

    (University of Houston
    University of Houston
    University of Houston)

  • Guillaume Luxardi

    (University of California)

  • Kan Zhu

    (University of California)

  • Min Zhao

    (University of California
    University of California)

Abstract

Redox state sustained by reactive oxygen species (ROS) is crucial for regeneration; however, the interplay between oxygen (O2), ROS and hypoxia-inducible factors (HIF) remains elusive. Here we observe, using an optic-based probe (optrode), an elevated and steady O2 influx immediately upon amputation. The spatiotemporal O2 influx profile correlates with the regeneration of Xenopus laevis tadpole tails. Inhibition of ROS production but not ROS scavenging decreases O2 influx. Inhibition of HIF-1α impairs regeneration and stabilization of HIF-1α induces regeneration in the refractory period. In the regeneration bud, hypoxia correlates with O2 influx, ROS production, and HIF-1α stabilization that modulate regeneration. Further analyses reveal that heat shock protein 90 is a putative downstream target of HIF-1α while electric current reversal is a de facto downstream target of HIF-1α. Collectively, the results show a mechanism for regeneration via the orchestration of O2 influx, ROS production, and HIF-1α stabilization.

Suggested Citation

  • Fernando Ferreira & VijayKrishna Raghunathan & Guillaume Luxardi & Kan Zhu & Min Zhao, 2018. "Early redox activities modulate Xenopus tail regeneration," Nature Communications, Nature, vol. 9(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-06614-2
    DOI: 10.1038/s41467-018-06614-2
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