IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v9y2018i1d10.1038_s41467-018-05912-z.html
   My bibliography  Save this article

Lamin B1 mapping reveals the existence of dynamic and functional euchromatin lamin B1 domains

Author

Listed:
  • Laura Pascual-Reguant

    (Vall d’Hebron Institute of Oncology)

  • Enrique Blanco

    (The Barcelona Institute of Science and Technology)

  • Silvia Galan

    (The Barcelona Institute of Science and Technology
    The Barcelona Institute of Science and Technology)

  • François Dily

    (The Barcelona Institute of Science and Technology
    Universitat Pompeu Fabra (UPF))

  • Yasmina Cuartero

    (The Barcelona Institute of Science and Technology
    The Barcelona Institute of Science and Technology)

  • Gemma Serra-Bardenys

    (Vall d’Hebron Institute of Oncology
    Universitat Pompeu Fabra (UPF))

  • Valerio Carlo

    (The Barcelona Institute of Science and Technology)

  • Ane Iturbide

    (Institute of Epigenetics and Stem Cells)

  • Joan Pau Cebrià-Costa

    (Vall d’Hebron Institute of Oncology)

  • Lara Nonell

    (Servei d’Anàlisi de Microarrays Institut Hospital del Mar d’Investigacions Mèdiques)

  • Antonio García Herreros

    (Universitat Pompeu Fabra (UPF)
    Institut Hospital del Mar d’Investigacions Mèdiques)

  • Luciano Croce

    (The Barcelona Institute of Science and Technology
    ICREA, Pg. Lluis Companys 23)

  • Marc A. Marti-Renom

    (The Barcelona Institute of Science and Technology
    The Barcelona Institute of Science and Technology
    Universitat Pompeu Fabra (UPF)
    ICREA, Pg. Lluis Companys 23)

  • Sandra Peiró

    (Vall d’Hebron Institute of Oncology)

Abstract

Lamins (A/C and B) are major constituents of the nuclear lamina (NL). Structurally conserved lamina-associated domains (LADs) are formed by genomic regions that contact the NL. Lamins are also found in the nucleoplasm, with a yet unknown function. Here we map the genome-wide localization of lamin B1 in an euchromatin-enriched fraction of the mouse genome and follow its dynamics during the epithelial-to-mesenchymal transition (EMT). Lamin B1 associates with actively expressed and open euchromatin regions, forming dynamic euchromatin lamin B1-associated domains (eLADs) of about 0.3 Mb. Hi-C data link eLADs to the 3D organization of the mouse genome during EMT and correlate lamin B1 enrichment at topologically associating domain (TAD) borders with increased border strength. Having reduced levels of lamin B1 alters the EMT transcriptional signature and compromises the acquisition of mesenchymal traits. Thus, during EMT, the process of genome reorganization in mouse involves dynamic changes in eLADs.

Suggested Citation

  • Laura Pascual-Reguant & Enrique Blanco & Silvia Galan & François Dily & Yasmina Cuartero & Gemma Serra-Bardenys & Valerio Carlo & Ane Iturbide & Joan Pau Cebrià-Costa & Lara Nonell & Antonio García He, 2018. "Lamin B1 mapping reveals the existence of dynamic and functional euchromatin lamin B1 domains," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05912-z
    DOI: 10.1038/s41467-018-05912-z
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-018-05912-z
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-018-05912-z?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05912-z. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.