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Variation in bradyrhizobial NopP effector determines symbiotic incompatibility with Rj2-soybeans via effector-triggered immunity

Author

Listed:
  • Masayuki Sugawara

    (Tohoku University)

  • Satoko Takahashi

    (Tohoku University)

  • Yosuke Umehara

    (National Agriculture and Food Research Organization)

  • Hiroya Iwano

    (Tohoku University)

  • Hirohito Tsurumaru

    (Kagoshima University)

  • Haruka Odake

    (Tohoku University)

  • Yuta Suzuki

    (Tohoku University)

  • Hitoshi Kondo

    (Tohoku University)

  • Yuki Konno

    (Tohoku University)

  • Takeo Yamakawa

    (Kyushu University)

  • Shusei Sato

    (Tohoku University)

  • Hisayuki Mitsui

    (Tohoku University)

  • Kiwamu Minamisawa

    (Tohoku University)

Abstract

Genotype-specific incompatibility in legume–rhizobium symbiosis has been suggested to be controlled by effector-triggered immunity underlying pathogenic host-bacteria interactions. However, the rhizobial determinant interacting with the host resistance protein (e.g., Rj2) and the molecular mechanism of symbiotic incompatibility remain unclear. Using natural mutants of Bradyrhizobium diazoefficiens USDA 122, we identified a type III-secretory protein NopP as the determinant of symbiotic incompatibility with Rj2-soybean. The analysis of nopP mutations and variants in a culture collection reveal that three amino acid residues (R60, R67, and H173) in NopP are required for Rj2-mediated incompatibility. Complementation of rj2-soybean by the Rj2 allele confers the incompatibility induced by USDA 122-type NopP. In response to incompatible strains, Rj2-soybean plants activate defense marker gene PR-2 and suppress infection thread number at 2 days after inoculation. These results suggest that Rj2-soybeans monitor the specific variants of NopP and reject bradyrhizobial infection via effector-triggered immunity mediated by Rj2 protein.

Suggested Citation

  • Masayuki Sugawara & Satoko Takahashi & Yosuke Umehara & Hiroya Iwano & Hirohito Tsurumaru & Haruka Odake & Yuta Suzuki & Hitoshi Kondo & Yuki Konno & Takeo Yamakawa & Shusei Sato & Hisayuki Mitsui & K, 2018. "Variation in bradyrhizobial NopP effector determines symbiotic incompatibility with Rj2-soybeans via effector-triggered immunity," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05663-x
    DOI: 10.1038/s41467-018-05663-x
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