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Size control in mammalian cells involves modulation of both growth rate and cell cycle duration

Author

Listed:
  • Clotilde Cadart

    (PSL Research University, CNRS, UMR 144
    PSL Research University)

  • Sylvain Monnier

    (PSL Research University, CNRS, UMR 144
    Univ. Lyon, Université Claude Bernard Lyon 1, CNRS, Institut Lumière Matière)

  • Jacopo Grilli

    (University of Chicago
    Santa Fe Institute)

  • Pablo J. Sáez

    (PSL Research University, CNRS, UMR 144
    PSL Research University)

  • Nishit Srivastava

    (PSL Research University, CNRS, UMR 144
    PSL Research University)

  • Rafaele Attia

    (PSL Research University, CNRS, UMR 144
    PSL Research University)

  • Emmanuel Terriac

    (PSL Research University, CNRS, UMR 144
    INM-Leibniz Institute for New Materials)

  • Buzz Baum

    (MRC Laboratory for Molecular Cell Biology, UCL
    Institute of Physics of Living Systems, UCL)

  • Marco Cosentino-Lagomarsino

    (Sorbonne Universités, Université Pierre et Marie Curie
    CNRS, UMR 7238 Computational and Quantitative Biology
    FIRC Institute of Molecular Oncology (IFOM))

  • Matthieu Piel

    (PSL Research University, CNRS, UMR 144
    PSL Research University)

Abstract

Despite decades of research, how mammalian cell size is controlled remains unclear because of the difficulty of directly measuring growth at the single-cell level. Here we report direct measurements of single-cell volumes over entire cell cycles on various mammalian cell lines and primary human cells. We find that, in a majority of cell types, the volume added across the cell cycle shows little or no correlation to cell birth size, a homeostatic behavior called “adder”. This behavior involves modulation of G1 or S-G2 duration and modulation of growth rate. The precise combination of these mechanisms depends on the cell type and the growth condition. We have developed a mathematical framework to compare size homeostasis in datasets ranging from bacteria to mammalian cells. This reveals that a near-adder behavior is the most common type of size control and highlights the importance of growth rate modulation to size control in mammalian cells.

Suggested Citation

  • Clotilde Cadart & Sylvain Monnier & Jacopo Grilli & Pablo J. Sáez & Nishit Srivastava & Rafaele Attia & Emmanuel Terriac & Buzz Baum & Marco Cosentino-Lagomarsino & Matthieu Piel, 2018. "Size control in mammalian cells involves modulation of both growth rate and cell cycle duration," Nature Communications, Nature, vol. 9(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-05393-0
    DOI: 10.1038/s41467-018-05393-0
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    Cited by:

    1. Chengjun Cao & Keyi Wang & Yina Wang & Tong-Bao Liu & Amariliz Rivera & Chaoyang Xue, 2022. "Ubiquitin proteolysis of a CDK-related kinase regulates titan cell formation and virulence in the fungal pathogen Cryptococcus neoformans," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    2. Klement Stojanovski & Ioana Gheorghe & Peter Lenart & Anne Lanjuin & William B. Mair & Benjamin D. Towbin, 2023. "Maintenance of appropriate size scaling of the C. elegans pharynx by YAP-1," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    3. Tim Lijster & Christoffer Åberg, 2020. "Asymmetry of nanoparticle inheritance upon cell division: Effect on the coefficient of variation," PLOS ONE, Public Library of Science, vol. 15(11), pages 1-18, November.
    4. Klement Stojanovski & Helge Großhans & Benjamin D. Towbin, 2022. "Coupling of growth rate and developmental tempo reduces body size heterogeneity in C. elegans," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    5. Fabrizio A. Pennacchio & Alessandro Poli & Francesca Michela Pramotton & Stefania Lavore & Ilaria Rancati & Mario Cinquanta & Daan Vorselen & Elisabetta Prina & Orso Maria Romano & Aldo Ferrari & Matt, 2024. "N2FXm, a method for joint nuclear and cytoplasmic volume measurements, unravels the osmo-mechanical regulation of nuclear volume in mammalian cells," Nature Communications, Nature, vol. 15(1), pages 1-10, December.

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