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Structural basis for terminal loop recognition and stimulation of pri-miRNA-18a processing by hnRNP A1

Author

Listed:
  • Hamed Kooshapur

    (Helmholtz Zentrum München
    Technical University of Munich)

  • Nila Roy Choudhury

    (University of Edinburgh)

  • Bernd Simon

    (European Molecular Biology Laboratory)

  • Max Mühlbauer

    (Technical University of Munich
    Technical University of Munich)

  • Alexander Jussupow

    (Technical University of Munich
    Technical University of Munich)

  • Noemi Fernandez

    (Western General Hospital)

  • Alisha N. Jones

    (Helmholtz Zentrum München
    Technical University of Munich)

  • Andre Dallmann

    (Helmholtz Zentrum München
    Technical University of Munich)

  • Frank Gabel

    (Institut Laue-Langevin
    IBS)

  • Carlo Camilloni

    (Technical University of Munich
    Technical University of Munich)

  • Gracjan Michlewski

    (University of Edinburgh
    Western General Hospital
    University of Edinburgh
    Zhejiang University)

  • Javier F. Caceres

    (Western General Hospital)

  • Michael Sattler

    (Helmholtz Zentrum München
    Technical University of Munich)

Abstract

Post-transcriptional mechanisms play a predominant role in the control of microRNA (miRNA) production. Recognition of the terminal loop of precursor miRNAs by RNA-binding proteins (RBPs) influences their processing; however, the mechanistic basis for how levels of individual or subsets of miRNAs are regulated is mostly unexplored. We previously showed that hnRNP A1, an RBP implicated in many aspects of RNA processing, acts as an auxiliary factor that promotes the Microprocessor-mediated processing of pri-mir-18a. Here, by using an integrative structural biology approach, we show that hnRNP A1 forms a 1:1 complex with pri-mir-18a where both RNA recognition motifs (RRMs) bind to cognate RNA sequence motifs in the terminal loop of pri-mir-18a. Terminal loop binding induces an allosteric destabilization of base-pairing in the pri-mir-18a stem that promotes its downstream processing. Our results highlight terminal loop RNA recognition by RBPs as a potential general principle of miRNA biogenesis and regulation.

Suggested Citation

  • Hamed Kooshapur & Nila Roy Choudhury & Bernd Simon & Max Mühlbauer & Alexander Jussupow & Noemi Fernandez & Alisha N. Jones & Andre Dallmann & Frank Gabel & Carlo Camilloni & Gracjan Michlewski & Javi, 2018. "Structural basis for terminal loop recognition and stimulation of pri-miRNA-18a processing by hnRNP A1," Nature Communications, Nature, vol. 9(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04871-9
    DOI: 10.1038/s41467-018-04871-9
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