IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v9y2018i1d10.1038_s41467-018-04706-7.html
   My bibliography  Save this article

NEK7 regulates dendrite morphogenesis in neurons via Eg5-dependent microtubule stabilization

Author

Listed:
  • Francisco Freixo

    (The Barcelona Institute of Science and Technology)

  • Paula Martinez Delgado

    (Molecular Biology Institute of Barcelona (IBMB-CSIC))

  • Yasmina Manso

    (University of Barcelona
    ISCIII
    Vall d’Hebron Institute of Research
    Institucio Catalana de Recerca i Estudis Avancats (ICREA))

  • Carlos Sánchez-Huertas

    (The Barcelona Institute of Science and Technology
    UMR5237 Montpellier)

  • Cristina Lacasa

    (The Barcelona Institute of Science and Technology)

  • Eduardo Soriano

    (University of Barcelona
    ISCIII
    Vall d’Hebron Institute of Research
    Institucio Catalana de Recerca i Estudis Avancats (ICREA))

  • Joan Roig

    (Molecular Biology Institute of Barcelona (IBMB-CSIC))

  • Jens Lüders

    (The Barcelona Institute of Science and Technology)

Abstract

Organization of microtubules into ordered arrays is best understood in mitotic systems, but remains poorly characterized in postmitotic cells such as neurons. By analyzing the cycling cell microtubule cytoskeleton proteome through expression profiling and targeted RNAi screening for candidates with roles in neurons, we have identified the mitotic kinase NEK7. We show that NEK7 regulates dendrite morphogenesis in vitro and in vivo. NEK7 kinase activity is required for dendrite growth and branching, as well as spine formation and morphology. NEK7 regulates these processes in part through phosphorylation of the kinesin Eg5/KIF11, promoting its accumulation on microtubules in distal dendrites. Here, Eg5 limits retrograde microtubule polymerization, which is inhibitory to dendrite growth and branching. Eg5 exerts this effect through microtubule stabilization, independent of its motor activity. This work establishes NEK7 as a general regulator of the microtubule cytoskeleton, controlling essential processes in both mitotic cells and postmitotic neurons.

Suggested Citation

  • Francisco Freixo & Paula Martinez Delgado & Yasmina Manso & Carlos Sánchez-Huertas & Cristina Lacasa & Eduardo Soriano & Joan Roig & Jens Lüders, 2018. "NEK7 regulates dendrite morphogenesis in neurons via Eg5-dependent microtubule stabilization," Nature Communications, Nature, vol. 9(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04706-7
    DOI: 10.1038/s41467-018-04706-7
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-018-04706-7
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-018-04706-7?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-04706-7. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.