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Transcriptomic signatures of NK cells suggest impaired responsiveness in HIV-1 infection and increased activity post-vaccination

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  • Margaret C. Costanzo

    (Walter Reed Army Institute of Research
    Henry M. Jackson Foundation for the Advancement of Military Medicine)

  • Dohoon Kim

    (Walter Reed Army Institute of Research
    Henry M. Jackson Foundation for the Advancement of Military Medicine)

  • Matthew Creegan

    (Walter Reed Army Institute of Research
    Henry M. Jackson Foundation for the Advancement of Military Medicine)

  • Kerri G. Lal

    (Walter Reed Army Institute of Research
    Henry M. Jackson Foundation for the Advancement of Military Medicine)

  • Julie A. Ake

    (Walter Reed Army Institute of Research)

  • Jeffrey R. Currier

    (Walter Reed Army Institute of Research)

  • Hendrik Streeck

    (Walter Reed Army Institute of Research
    Henry M. Jackson Foundation for the Advancement of Military Medicine
    University Duisburg-Essen)

  • Merlin L. Robb

    (Walter Reed Army Institute of Research
    Henry M. Jackson Foundation for the Advancement of Military Medicine)

  • Nelson L. Michael

    (Walter Reed Army Institute of Research)

  • Diane L. Bolton

    (Walter Reed Army Institute of Research
    Henry M. Jackson Foundation for the Advancement of Military Medicine)

  • Nicholas J. Steers

    (Columbia University Medical Center)

  • Michael A. Eller

    (Walter Reed Army Institute of Research
    Henry M. Jackson Foundation for the Advancement of Military Medicine)

Abstract

Natural killer (NK) cells limit viral replication by direct recognition of infected cells, antibody-dependent cellular cytotoxicity (ADCC), and releasing cytokines. Although growing evidence supports NK cell antiviral immunity in HIV-1 infection, further knowledge of their response is necessary. Here we show that NK cells responding to models of direct cell recognition, ADCC, and cytokine activation have unique transcriptional fingerprints. Compared with healthy volunteers, individuals with chronic HIV-1 infection have higher expression of genes commonly associated with activation, and lower expression of genes associated with direct cell recognition and cytokine stimulation in their NK cells. By contrast, NK cell transcriptional profiles of individuals receiving a modified vaccinia Ankara (MVA) vectored HIV-1 vaccine show upregulation of genes associated with direct cell recognition. These findings demonstrate that targeted transcriptional profiling provides a sensitive assessment of NK cell activity, which helps understand how NK cells respond to viral infections and vaccination.

Suggested Citation

  • Margaret C. Costanzo & Dohoon Kim & Matthew Creegan & Kerri G. Lal & Julie A. Ake & Jeffrey R. Currier & Hendrik Streeck & Merlin L. Robb & Nelson L. Michael & Diane L. Bolton & Nicholas J. Steers & M, 2018. "Transcriptomic signatures of NK cells suggest impaired responsiveness in HIV-1 infection and increased activity post-vaccination," Nature Communications, Nature, vol. 9(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03618-w
    DOI: 10.1038/s41467-018-03618-w
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