IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v9y2018i1d10.1038_s41467-018-03432-4.html
   My bibliography  Save this article

Structural basis for the recognition of LDL-receptor family members by VSV glycoprotein

Author

Listed:
  • Jovan Nikolic

    (Université Paris-Saclay)

  • Laura Belot

    (Université Paris-Saclay)

  • Hélène Raux

    (Université Paris-Saclay)

  • Pierre Legrand

    (Synchrotron SOLEIL)

  • Yves Gaudin

    (Université Paris-Saclay)

  • Aurélie Albertini

    (Université Paris-Saclay)

Abstract

Vesicular stomatitis virus (VSV) is an oncolytic rhabdovirus and its glycoprotein G is widely used to pseudotype other viruses for gene therapy. Low-density lipoprotein receptor (LDL-R) serves as a major entry receptor for VSV. Here we report two crystal structures of VSV G in complex with two distinct cysteine-rich domains (CR2 and CR3) of LDL-R, showing that their binding sites on G are identical. We identify two basic residues on G, which are essential for its interaction with CR2 and CR3. Mutating these residues abolishes VSV infectivity even though VSV can use alternative receptors, indicating that all VSV receptors are members of the LDL-R family. Collectively, our data suggest that VSV G has specifically evolved to interact with receptor CR domains. These structural insights into the interaction between VSV G and host cell receptors provide a basis for the design of recombinant viruses with an altered tropism.

Suggested Citation

  • Jovan Nikolic & Laura Belot & Hélène Raux & Pierre Legrand & Yves Gaudin & Aurélie Albertini, 2018. "Structural basis for the recognition of LDL-receptor family members by VSV glycoprotein," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03432-4
    DOI: 10.1038/s41467-018-03432-4
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-018-03432-4
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-018-03432-4?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Xiaofeng Zhai & Xiaoling Li & Michael Veit & Ningning Wang & Yu Wang & Andres Merits & Zhiwen Jiang & Yan Qin & Xiaoguang Zhang & Kaili Qi & Houqi Jiao & Wan-Ting He & Ye Chen & Yang Mao & Shuo Su, 2024. "LDLR is used as a cell entry receptor by multiple alphaviruses," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    2. Daniel Strebinger & Chris J. Frangieh & Mirco J. Friedrich & Guilhem Faure & Rhiannon K. Macrae & Feng Zhang, 2023. "Cell type-specific delivery by modular envelope design," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    3. Maureen Ritter & Lola Canus & Anupriya Gautam & Thomas Vallet & Li Zhong & Alexandre Lalande & Bertrand Boson & Apoorv Gandhi & Sergueï Bodoirat & Julien Burlaud-Gaillard & Natalia Freitas & Philippe , 2024. "The low-density lipoprotein receptor and apolipoprotein E associated with CCHFV particles mediate CCHFV entry into cells," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    4. Hongming Ma & Lucas J. Adams & Saravanan Raju & Alan Sariol & Natasha M. Kafai & Hana Janova & William B. Klimstra & Daved H. Fremont & Michael S. Diamond, 2024. "The low-density lipoprotein receptor promotes infection of multiple encephalitic alphaviruses," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    5. Cesare Canepari & Michela Milani & Chiara Simoni & Francesco Starinieri & Monica Volpin & Anna Fabiano & Mauro Biffi & Fabio Russo & Rossana Norata & Martina Rocchi & Chiara Brombin & Federica Cugnata, 2025. "Enhancing the potency of in vivo lentiviral vector mediated gene therapy to hepatocytes," Nature Communications, Nature, vol. 16(1), pages 1-17, December.
    6. Pan Yang & Wanyu Li & Xiaoyi Fan & Junhua Pan & Colin J. Mann & Haley Varnum & Lars E. Clark & Sarah A. Clark & Adrian Coscia & Himanish Basu & Katherine Nabel Smith & Vesna Brusic & Jonathan Abraham, 2024. "Structural basis for VLDLR recognition by eastern equine encephalitis virus," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    7. Xiuming Liang & Dhanu Gupta & Junhua Xie & Elien Wonterghem & Lien Hoecke & Justin Hean & Zheyu Niu & Marziyeh Ghaeidamini & Oscar P. B. Wiklander & Wenyi Zheng & Rim Jawad Wiklander & Rui He & Doste , 2025. "Engineering of extracellular vesicles for efficient intracellular delivery of multimodal therapeutics including genome editors," Nature Communications, Nature, vol. 16(1), pages 1-18, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03432-4. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.