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A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging

Author

Listed:
  • David Virant

    (Max Planck Institute for Terrestrial Microbiology and LOEWE Center for Synthetic Microbiology (SYNMIKRO))

  • Bjoern Traenkle

    (Eberhard Karls University Tuebingen)

  • Julia Maier

    (Eberhard Karls University Tuebingen)

  • Philipp D. Kaiser

    (Natural and Medical Sciences Institute at the University of Tuebingen)

  • Mona Bodenhöfer

    (Natural and Medical Sciences Institute at the University of Tuebingen)

  • Christian Schmees

    (Natural and Medical Sciences Institute at the University of Tuebingen)

  • Ilijana Vojnovic

    (Max Planck Institute for Terrestrial Microbiology and LOEWE Center for Synthetic Microbiology (SYNMIKRO))

  • Borbála Pisak-Lukáts

    (Max Planck Institute for Terrestrial Microbiology and LOEWE Center for Synthetic Microbiology (SYNMIKRO))

  • Ulrike Endesfelder

    (Max Planck Institute for Terrestrial Microbiology and LOEWE Center for Synthetic Microbiology (SYNMIKRO))

  • Ulrich Rothbauer

    (Eberhard Karls University Tuebingen
    Natural and Medical Sciences Institute at the University of Tuebingen)

Abstract

Dense fluorophore labeling without compromising the biological target is crucial for genuine super-resolution microscopy. Here we introduce a broadly applicable labeling strategy for fixed and living cells utilizing a short peptide tag-specific nanobody (BC2-tag/bivBC2-Nb). BC2-tagging of ectopically introduced or endogenous proteins does not interfere with the examined structures and bivBC2-Nb staining results in a close-grained fluorophore labeling with minimal linkage errors. This allowed us to perform high-quality dSTORM imaging of various targets in mammalian and yeast cells. We expect that this versatile strategy will render many more demanding cellular targets amenable to dSTORM imaging.

Suggested Citation

  • David Virant & Bjoern Traenkle & Julia Maier & Philipp D. Kaiser & Mona Bodenhöfer & Christian Schmees & Ilijana Vojnovic & Borbála Pisak-Lukáts & Ulrike Endesfelder & Ulrich Rothbauer, 2018. "A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging," Nature Communications, Nature, vol. 9(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03191-2
    DOI: 10.1038/s41467-018-03191-2
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