IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v9y2018i1d10.1038_s41467-018-02818-8.html
   My bibliography  Save this article

Membrane bending occurs at all stages of clathrin-coat assembly and defines endocytic dynamics

Author

Listed:
  • Brandon L. Scott

    (RM 131 South Dakota State University (SDSU)
    SDSU)

  • Kem A. Sochacki

    (National Institutes of Health)

  • Shalini T. Low-Nam

    (RM 131 South Dakota State University (SDSU)
    SDSU
    University of California)

  • Elizabeth M. Bailey

    (RM 131 South Dakota State University (SDSU)
    SDSU)

  • QuocAhn Luu

    (South Dakota School of Mines and Technology (SDSMT)
    SDSMT)

  • Amy Hor

    (South Dakota School of Mines and Technology (SDSMT)
    SDSMT)

  • Andrea M. Dickey

    (National Institutes of Health)

  • Steve Smith

    (South Dakota School of Mines and Technology (SDSMT)
    SDSMT)

  • Jason G. Kerkvliet

    (RM 131 South Dakota State University (SDSU)
    SDSU)

  • Justin W. Taraska

    (National Institutes of Health)

  • Adam D. Hoppe

    (RM 131 South Dakota State University (SDSU)
    SDSU)

Abstract

Clathrin-mediated endocytosis (CME) internalizes plasma membrane by reshaping small regions of the cell surface into spherical vesicles. The key mechanistic question of how coat assembly produces membrane curvature has been studied with molecular and cellular structural biology approaches, without direct visualization of the process in living cells; resulting in two competing models for membrane bending. Here we use polarized total internal reflection fluorescence microscopy (pol-TIRF) combined with electron, atomic force, and super-resolution optical microscopy to measure membrane curvature during CME. Surprisingly, coat assembly accommodates membrane bending concurrent with or after the assembly of the clathrin lattice. Once curvature began, CME proceeded to scission with robust timing. Four color pol-TIRF showed that CALM accumulated at high levels during membrane bending, implicating its auxiliary role in curvature generation. We conclude that clathrin-coat assembly is versatile and that multiple membrane-bending trajectories likely reflect the energetics of coat assembly relative to competing forces.

Suggested Citation

  • Brandon L. Scott & Kem A. Sochacki & Shalini T. Low-Nam & Elizabeth M. Bailey & QuocAhn Luu & Amy Hor & Andrea M. Dickey & Steve Smith & Jason G. Kerkvliet & Justin W. Taraska & Adam D. Hoppe, 2018. "Membrane bending occurs at all stages of clathrin-coat assembly and defines endocytic dynamics," Nature Communications, Nature, vol. 9(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-02818-8
    DOI: 10.1038/s41467-018-02818-8
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-018-02818-8
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-018-02818-8?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Tomasz J. Nawara & Yancey D. Williams & Tejeshwar C. Rao & Yuesong Hu & Elizabeth Sztul & Khalid Salaita & Alexa L. Mattheyses, 2022. "Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    2. Changsong Yang & Patricia Colosi & Siewert Hugelier & Daniel Zabezhinsky & Melike Lakadamyali & Tatyana Svitkina, 2022. "Actin polymerization promotes invagination of flat clathrin-coated lattices in mammalian cells by pushing at lattice edges," Nature Communications, Nature, vol. 13(1), pages 1-20, December.
    3. Kazuki Obashi & Kem A. Sochacki & Marie-Paule Strub & Justin W. Taraska, 2023. "A conformational switch in clathrin light chain regulates lattice structure and endocytosis at the plasma membrane of mammalian cells," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-02818-8. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.