IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v9y2018i1d10.1038_s41467-017-02741-4.html
   My bibliography  Save this article

Structure of a MacAB-like efflux pump from Streptococcus pneumoniae

Author

Listed:
  • Hong-Bo Yang

    (University of Science and Technology of China
    Chinese Academy of Science)

  • Wen-Tao Hou

    (University of Science and Technology of China
    Chinese Academy of Science)

  • Meng-Ting Cheng

    (University of Science and Technology of China
    Chinese Academy of Science)

  • Yong-Liang Jiang

    (University of Science and Technology of China
    Chinese Academy of Science)

  • Yuxing Chen

    (University of Science and Technology of China
    Chinese Academy of Science)

  • Cong-Zhao Zhou

    (University of Science and Technology of China
    Chinese Academy of Science)

Abstract

The spr0693-spr0694-spr0695 operon of Streptococcus pneumoniae encodes a putative ATP-binding cassette (ABC)-type efflux pump involved in the resistance of antibiotics and antimicrobial peptides. Here we report the crystal structures of Spr0694–0695 at 3.3 Å and Spr0693 at 3.0 Å resolution, revealing a MacAB-like efflux pump. The dimeric Spr0694–0695 adopts a non-canonical fold of ABC transporter, the transmembrane domain of which consists of eight tightly packed transmembrane helices with an insertion of extracellular domain between the first and second helices, whereas Spr0693 forms a nanotube channel docked onto the ABC transporter. Structural analyses combined with ATPase activity and antimicrobial susceptibility assays, enable us to propose a putative substrate-entrance tunnel with a lateral access controlled by a guard helix. Altogether, our findings provide structural insights and putative transport mechanism of a MacAB-like efflux pump in Gram-positive bacteria.

Suggested Citation

  • Hong-Bo Yang & Wen-Tao Hou & Meng-Ting Cheng & Yong-Liang Jiang & Yuxing Chen & Cong-Zhao Zhou, 2018. "Structure of a MacAB-like efflux pump from Streptococcus pneumoniae," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-017-02741-4
    DOI: 10.1038/s41467-017-02741-4
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-017-02741-4
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-017-02741-4?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-017-02741-4. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.