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RNAs coordinate nuclear envelope assembly and DNA replication through ELYS recruitment to chromatin

Author

Listed:
  • Antoine Aze

    (Institute of Human Genetics, UMR 9002, CNRS and the University of Montpellier, Replication and Genome Dynamics, 141 rue de la Cardonille)

  • Michalis Fragkos

    (Institute of Human Genetics, UMR 9002, CNRS and the University of Montpellier, Replication and Genome Dynamics, 141 rue de la Cardonille
    Institut Gustave Roussy, Genetic Stability and Oncogenesis Department, 39 rue Camille Desmoulins)

  • Stéphane Bocquet

    (Institute of Human Genetics, UMR 9002, CNRS and the University of Montpellier, Replication and Genome Dynamics, 141 rue de la Cardonille)

  • Julien Cau

    (Institute of Human Genetics, UMR 9002, CNRS and the University of Montpellier, Montpellier RIO Imaging, 141 rue de la Cardonille)

  • Marcel Méchali

    (Institute of Human Genetics, UMR 9002, CNRS and the University of Montpellier, Replication and Genome Dynamics, 141 rue de la Cardonille)

Abstract

Upon fertilisation, the sperm pronucleus acquires the competence to replicate the genome through a cascade of events that link chromatin remodelling to nuclear envelope formation. The factors involved have been partially identified and are poorly characterised. Here, using Xenopus laevis egg extracts we show that RNAs are required for proper nuclear envelope assembly following sperm DNA decondensation. Although chromatin remodelling and pre-replication complex formation occur normally, RNA-depleted extracts show a defect in pre-RC activation. The nuclear processes affected by RNA-depletion included ELYS recruitment, which accounts for the deficiency in nuclear pore complex assembly. This results in failure in chromatin relaxation as well as in the import and proper nuclear concentration of the S-phase kinases necessary for DNA replication activation. Our results highlight a translation-independent RNA function necessary for the parental genome progression towards the early embryonic cell cycle programme.

Suggested Citation

  • Antoine Aze & Michalis Fragkos & Stéphane Bocquet & Julien Cau & Marcel Méchali, 2017. "RNAs coordinate nuclear envelope assembly and DNA replication through ELYS recruitment to chromatin," Nature Communications, Nature, vol. 8(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-02180-1
    DOI: 10.1038/s41467-017-02180-1
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    Cited by:

    1. Aina Maria Mas & Enrique Goñi & Igor Ruiz de los Mozos & Aida Arcas & Luisa Statello & Jovanna González & Lorea Blázquez & Wei Ting Chelsea Lee & Dipika Gupta & Álvaro Sejas & Shoko Hoshina & Alexandr, 2023. "ORC1 binds to cis-transcribed RNAs for efficient activation of replication origins," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

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