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Mass spectrometry sequencing of long digital polymers facilitated by programmed inter-byte fragmentation

Author

Listed:
  • Abdelaziz Al Ouahabi

    (Institut Charles Sadron UPR22)

  • Jean-Arthur Amalian

    (Institute of Radical Chemistry)

  • Laurence Charles

    (Institute of Radical Chemistry)

  • Jean-François Lutz

    (Institut Charles Sadron UPR22)

Abstract

In the context of data storage miniaturization, it was recently shown that digital information can be stored in the monomer sequences of non-natural macromolecules. However, the sequencing of such digital polymers is currently limited to short chains. Here, we report that intact multi-byte digital polymers can be sequenced in a moderate resolution mass spectrometer and that full sequence coverage can be attained without requiring pre-analysis digestion or the help of sequence databases. In order to do so, the polymers are designed to undergo controlled fragmentations in collision-induced dissociation conditions. Each byte of the sequence is labeled by an identification tag and a weak alkoxyamine group is placed between 2 bytes. As a consequence of this design, the NO-C bonds break first upon collisional activation, thus leading to a pattern of mass tag-shifted intact bytes. Afterwards, each byte is individually sequenced in pseudo-MS3 conditions and the whole sequence is found.

Suggested Citation

  • Abdelaziz Al Ouahabi & Jean-Arthur Amalian & Laurence Charles & Jean-François Lutz, 2017. "Mass spectrometry sequencing of long digital polymers facilitated by programmed inter-byte fragmentation," Nature Communications, Nature, vol. 8(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01104-3
    DOI: 10.1038/s41467-017-01104-3
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