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A structural model of flagellar filament switching across multiple bacterial species

Author

Listed:
  • Fengbin Wang

    (University of Virginia School of Medicine)

  • Andrew M. Burrage

    (Indiana University)

  • Sandra Postel

    (Institute of Human Virology and University of Maryland School of Medicine)

  • Reece E. Clark

    (Indiana University)

  • Albina Orlova

    (University of Virginia School of Medicine)

  • Eric J. Sundberg

    (Institute of Human Virology and University of Maryland School of Medicine
    University of Maryland School of Medicine)

  • Daniel B. Kearns

    (Indiana University)

  • Edward H. Egelman

    (University of Virginia School of Medicine)

Abstract

The bacterial flagellar filament has long been studied to understand how a polymer composed of a single protein can switch between different supercoiled states with high cooperativity. Here we present near-atomic resolution cryo-EM structures for flagellar filaments from both Gram-positive Bacillus subtilis and Gram-negative Pseudomonas aeruginosa. Seven mutant flagellar filaments in B. subtilis and two in P. aeruginosa capture two different states of the filament. These reliable atomic models of both states reveal conserved molecular interactions in the interior of the filament among B. subtilis, P. aeruginosa and Salmonella enterica. Using the detailed information about the molecular interactions in two filament states, we successfully predict point mutations that shift the equilibrium between those two states. Further, we observe the dimerization of P. aeruginosa outer domains without any perturbation of the conserved interior of the filament. Our results give new insights into how the flagellin sequence has been “tuned” over evolution.

Suggested Citation

  • Fengbin Wang & Andrew M. Burrage & Sandra Postel & Reece E. Clark & Albina Orlova & Eric J. Sundberg & Daniel B. Kearns & Edward H. Egelman, 2017. "A structural model of flagellar filament switching across multiple bacterial species," Nature Communications, Nature, vol. 8(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01075-5
    DOI: 10.1038/s41467-017-01075-5
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    Cited by:

    1. Nicholas M Thomson & Josie L Ferreira & Teige R Matthews-Palmer & Morgan Beeby & Mark J Pallen, 2018. "Giant flagellins form thick flagellar filaments in two species of marine γ-proteobacteria," PLOS ONE, Public Library of Science, vol. 13(11), pages 1-12, November.

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