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PD-L1 is an activation-independent marker of brown adipocytes

Author

Listed:
  • Jessica R. Ingram

    (Whitehead Institute for Biomedical Research
    Dana-Farber Cancer Institute)

  • Michael Dougan

    (Whitehead Institute for Biomedical Research
    Massachusetts General Hospital)

  • Mohammad Rashidian

    (Whitehead Institute for Biomedical Research)

  • Marko Knoll

    (Whitehead Institute for Biomedical Research)

  • Edmund J. Keliher

    (Massachusetts General Hospital
    Massachusetts General Hospital)

  • Sarah Garrett

    (Albert Einstein College of Medicine)

  • Scott Garforth

    (Albert Einstein College of Medicine)

  • Olga S. Blomberg

    (Whitehead Institute for Biomedical Research)

  • Camilo Espinosa

    (Whitehead Institute for Biomedical Research)

  • Atul Bhan

    (Massachusetts General Hospital)

  • Steven C. Almo

    (Albert Einstein College of Medicine)

  • Ralph Weissleder

    (Massachusetts General Hospital
    Massachusetts General Hospital
    Harvard Medical School)

  • Harvey Lodish

    (Whitehead Institute for Biomedical Research
    Massachusetts Institute of Technology)

  • Stephanie K. Dougan

    (Dana-Farber Cancer Institute
    Harvard Medical School)

  • Hidde L. Ploegh

    (Whitehead Institute for Biomedical Research
    Massachusetts Institute of Technology)

Abstract

Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state.

Suggested Citation

  • Jessica R. Ingram & Michael Dougan & Mohammad Rashidian & Marko Knoll & Edmund J. Keliher & Sarah Garrett & Scott Garforth & Olga S. Blomberg & Camilo Espinosa & Atul Bhan & Steven C. Almo & Ralph Wei, 2017. "PD-L1 is an activation-independent marker of brown adipocytes," Nature Communications, Nature, vol. 8(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00799-8
    DOI: 10.1038/s41467-017-00799-8
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