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Noise reduction as an emergent property of single-cell aging

Author

Listed:
  • Ping Liu

    (Yale University
    Yale University)

  • Ruijie Song

    (Yale University
    Yale University)

  • Gregory L. Elison

    (Yale University
    Yale University)

  • Weilin Peng

    (Yale University
    Yale University)

  • Murat Acar

    (Yale University
    Yale University
    Yale University
    Yale University)

Abstract

Noise-induced heterogeneity in gene expression is an inherent reality for cells. However, it is not well understood how noise strength changes for a single gene while the host cell is aging. Using a state-of-the-art microfluidic platform, we measure noise dynamics in aging yeast cells by tracking the generation-specific activity of the canonical GAL1 promoter. We observe noise reduction during normal aging of a cell, followed by a short catastrophe phase in which noise increased. We hypothesize that aging-associated increases in chromatin state transitions are behind the observed noise reduction and a stochastic model provides quantitative support to the proposed mechanism. Noise trends measured from strains with altered GAL1 promoter dynamics (constitutively active, synthetic with nucleosome-disfavoring sequences, and in the absence of RPD3, a global remodeling regulator) lend further support to our hypothesis. Observing similar noise dynamics from a different promoter (HHF2) provides support to the generality of our findings.

Suggested Citation

  • Ping Liu & Ruijie Song & Gregory L. Elison & Weilin Peng & Murat Acar, 2017. "Noise reduction as an emergent property of single-cell aging," Nature Communications, Nature, vol. 8(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00752-9
    DOI: 10.1038/s41467-017-00752-9
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