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Biochemical phosphates observed using hyperpolarized 31P in physiological aqueous solutions

Author

Listed:
  • Atara Nardi-Schreiber

    (Hadassah-Hebrew University Medical Center)

  • Ayelet Gamliel

    (Hadassah-Hebrew University Medical Center)

  • Talia Harris

    (Hadassah-Hebrew University Medical Center)

  • Gal Sapir

    (Hadassah-Hebrew University Medical Center)

  • Jacob Sosna

    (Hadassah-Hebrew University Medical Center)

  • J. Moshe Gomori

    (Hadassah-Hebrew University Medical Center)

  • Rachel Katz-Brull

    (Hadassah-Hebrew University Medical Center)

Abstract

The dissolution-dynamic nuclear polarization technology had previously enabled nuclear magnetic resonance detection of various nuclei in a hyperpolarized state. Here, we show the hyperpolarization of 31P nuclei in important biological phosphates (inorganic phosphate and phosphocreatine) in aqueous solutions. The hyperpolarized inorganic phosphate showed an enhancement factor >11,000 (at 5.8 T, 9.3% polarization) in D2O (T1 29.4 s). Deuteration and the solution composition and pH all affected the lifetime of the hyperpolarized state. This capability opens up avenues for real-time monitoring of phosphate metabolism, distribution, and pH sensing in the live body without ionizing radiation. Immediate changes in the microenvironment pH have been detected here in a cell-free system via the chemical shift of hyperpolarized inorganic phosphate. Because the 31P nucleus is 100% naturally abundant, future studies on hyperpolarized phosphates will not require expensive isotope labeling as is usually required for hyperpolarization of other substrates.

Suggested Citation

  • Atara Nardi-Schreiber & Ayelet Gamliel & Talia Harris & Gal Sapir & Jacob Sosna & J. Moshe Gomori & Rachel Katz-Brull, 2017. "Biochemical phosphates observed using hyperpolarized 31P in physiological aqueous solutions," Nature Communications, Nature, vol. 8(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00364-3
    DOI: 10.1038/s41467-017-00364-3
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