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Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping

Author

Listed:
  • Stefanie L. Morgan

    (Program in Epithelial Biology, Stanford University School of Medicine
    Program in Cancer Biology, Stanford University School of Medicine)

  • Natasha C. Mariano

    (Program in Epithelial Biology, Stanford University School of Medicine
    Program in Cancer Biology, Stanford University School of Medicine)

  • Abel Bermudez

    (Canary Center for Cancer Early Detection, Stanford University School of Medicine)

  • Nicole L. Arruda

    (Bridgewater State University)

  • Fangting Wu

    (System Biosciences)

  • Yunhai Luo

    (Program in Epithelial Biology, Stanford University School of Medicine
    Program in Cancer Biology, Stanford University School of Medicine)

  • Gautam Shankar

    (Program in Epithelial Biology, Stanford University School of Medicine
    Program in Cancer Biology, Stanford University School of Medicine)

  • Lin Jia

    (Veterans Affairs Healthcare System
    Stem Cell and Cancer Center, First Affiliated Hospital, Jilin University)

  • Huiling Chen

    (Veterans Affairs Healthcare System
    Xiangya Hospital, Central South University)

  • Ji-Fan Hu

    (Veterans Affairs Healthcare System
    Stem Cell and Cancer Center, First Affiliated Hospital, Jilin University)

  • Andrew R. Hoffman

    (Veterans Affairs Healthcare System)

  • Chiao-Chain Huang

    (System Biosciences)

  • Sharon J. Pitteri

    (Canary Center for Cancer Early Detection, Stanford University School of Medicine)

  • Kevin C. Wang

    (Program in Epithelial Biology, Stanford University School of Medicine
    Program in Cancer Biology, Stanford University School of Medicine
    Veterans Affairs Healthcare System)

Abstract

Chromatin looping is key to gene regulation, yet no broadly applicable methods to selectively modify chromatin loops have been described. We have engineered a method for chromatin loop reorganization using CRISPR-dCas9 (CLOuD9) to selectively and reversibly establish chromatin loops. We demonstrate the power of this technology to selectively modulate gene expression at targeted loci.

Suggested Citation

  • Stefanie L. Morgan & Natasha C. Mariano & Abel Bermudez & Nicole L. Arruda & Fangting Wu & Yunhai Luo & Gautam Shankar & Lin Jia & Huiling Chen & Ji-Fan Hu & Andrew R. Hoffman & Chiao-Chain Huang & Sh, 2017. "Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping," Nature Communications, Nature, vol. 8(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15993
    DOI: 10.1038/ncomms15993
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    Cited by:

    1. Irene Robles-Rebollo & Sergi Cuartero & Adria Canellas-Socias & Sarah Wells & Mohammad M. Karimi & Elisabetta Mereu & Alexandra G. Chivu & Holger Heyn & Chad Whilding & Dirk Dormann & Samuel Marguerat, 2022. "Cohesin couples transcriptional bursting probabilities of inducible enhancers and promoters," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    2. Manyu Du & Fan Zou & Yi Li & Yujie Yan & Lu Bai, 2022. "Chemically Induced Chromosomal Interaction (CICI) method to study chromosome dynamics and its biological roles," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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