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USP13 regulates the RAP80-BRCA1 complex dependent DNA damage response

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  • Yunhui Li

    (Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine
    Key Laboratory of Arrhythmias of the Ministry of Education of China, East Hospital, Tongji University School of Medicine)

  • Kuntian Luo

    (Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine
    Key Laboratory of Arrhythmias of the Ministry of Education of China, East Hospital, Tongji University School of Medicine
    Mayo Clinic)

  • Yujiao Yin

    (Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine
    Key Laboratory of Arrhythmias of the Ministry of Education of China, East Hospital, Tongji University School of Medicine)

  • Chenming Wu

    (Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine
    Key Laboratory of Arrhythmias of the Ministry of Education of China, East Hospital, Tongji University School of Medicine)

  • Min Deng

    (Mayo Clinic)

  • Lei Li

    (Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine
    Key Laboratory of Arrhythmias of the Ministry of Education of China, East Hospital, Tongji University School of Medicine)

  • Yuping Chen

    (Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine
    Key Laboratory of Arrhythmias of the Ministry of Education of China, East Hospital, Tongji University School of Medicine)

  • Somaira Nowsheen

    (Medical Scientist Training Program, Mayo Clinic School of Medicine, Mayo Clinic Graduate School of Biomedical Sciences)

  • Zhenkun Lou

    (Mayo Clinic)

  • Jian Yuan

    (Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine
    Key Laboratory of Arrhythmias of the Ministry of Education of China, East Hospital, Tongji University School of Medicine
    Mayo Clinic)

Abstract

BRCA1 regulates multiple cellular pathways that maintain genomic stability including cell cycle checkpoints, DNA repair, protein ubiquitination, chromatin remodelling, transcriptional regulation and apoptosis. Receptor-associated protein 80 (RAP80) helps recruit BRCA1 to double-strand breaks (DSBs) through the scaffold protein CCDC98 (Abraxas) and facilitates DNA damage response (DDR). However, the regulation of RAP80-BRCA1 complex is still unclear. Here we report that a deubiquitinase, USP13, regulates DDR by targeting RAP80. Mechanistically, USP13 is phosphorylated by ATM following DNA damage which, in turn, facilitates its DSB localization. USP13, in turn, deubiquitinates RAP80 and promotes RAP80 recruitment and proper DDR. Depleting or inhibiting USP13 sensitizes ovarian cancer cells to cisplatin and PARP inhibitor (olaparib) while overexpression of USP13 renders ovarian cancer cells resistant to chemotherapy. Overall, we identify USP13 as a regulator of DNA repair and reveal a model in which a phosphorylation-deubiquitination axis dynamically regulates RAP80-BRCA1 complex foci formation and function.

Suggested Citation

  • Yunhui Li & Kuntian Luo & Yujiao Yin & Chenming Wu & Min Deng & Lei Li & Yuping Chen & Somaira Nowsheen & Zhenkun Lou & Jian Yuan, 2017. "USP13 regulates the RAP80-BRCA1 complex dependent DNA damage response," Nature Communications, Nature, vol. 8(1), pages 1-12, August.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15752
    DOI: 10.1038/ncomms15752
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