IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_ncomms15055.html
   My bibliography  Save this article

Direct comparison of distinct naive pluripotent states in human embryonic stem cells

Author

Listed:
  • S. Warrier

    (Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital)

  • M. Van der Jeught

    (Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital)

  • G. Duggal

    (Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital
    John Radcliffe Hospital, University of Oxford)

  • L. Tilleman

    (Laboratory for Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University)

  • E. Sutherland

    (Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital)

  • J. Taelman

    (Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital)

  • M. Popovic

    (Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital)

  • S. Lierman

    (Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital)

  • S. Chuva De Sousa Lopes

    (Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital
    Leiden University Medical Center)

  • A. Van Soom

    (Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University)

  • L. Peelman

    (Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University)

  • F. Van Nieuwerburgh

    (Laboratory for Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University)

  • D. I. M. De Coninck

    (Laboratory for Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University)

  • B. Menten

    (Center for Medical Genetics, Ghent University Hospital)

  • P. Mestdagh

    (Center for Medical Genetics, Ghent University Hospital)

  • J. Van de Sompele

    (Center for Medical Genetics, Ghent University Hospital)

  • D. Deforce

    (Laboratory for Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University)

  • P. De Sutter

    (Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital)

  • B. Heindryckx

    (Ghent-Fertility and Stem cell Team (G-FaST), Ghent University Hospital)

Abstract

Until recently, human embryonic stem cells (hESCs) were shown to exist in a state of primed pluripotency, while mouse embryonic stem cells (mESCs) display a naive or primed pluripotent state. Here we show the rapid conversion of in-house-derived primed hESCs on mouse embryonic feeder layer (MEF) to a naive state within 5–6 days in naive conversion media (NCM-MEF), 6–10 days in naive human stem cell media (NHSM-MEF) and 14–20 days using the reverse-toggle protocol (RT-MEF). We further observe enhanced unbiased lineage-specific differentiation potential of naive hESCs converted in NCM-MEF, however, all naive hESCs fail to differentiate towards functional cell types. RNA-seq analysis reveals a divergent role of PI3K/AKT/mTORC signalling, specifically of the mTORC2 subunit, in the different naive hESCs. Overall, we demonstrate a direct evaluation of several naive culture conditions performed in the same laboratory, thereby contributing to an unbiased, more in-depth understanding of different naive hESCs.

Suggested Citation

  • S. Warrier & M. Van der Jeught & G. Duggal & L. Tilleman & E. Sutherland & J. Taelman & M. Popovic & S. Lierman & S. Chuva De Sousa Lopes & A. Van Soom & L. Peelman & F. Van Nieuwerburgh & D. I. M. De, 2017. "Direct comparison of distinct naive pluripotent states in human embryonic stem cells," Nature Communications, Nature, vol. 8(1), pages 1-10, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15055
    DOI: 10.1038/ncomms15055
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms15055
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms15055?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15055. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.