IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_ncomms14591.html
   My bibliography  Save this article

Ensemble and single-molecule dynamics of IFT dynein in Caenorhabditis elegans cilia

Author

Listed:
  • Jona Mijalkovic

    (Vrije Universiteit Amsterdam)

  • Bram Prevo

    (Vrije Universiteit Amsterdam)

  • Felix Oswald

    (Vrije Universiteit Amsterdam)

  • Pierre Mangeol

    (Vrije Universiteit Amsterdam
    Present address: Developmental Biology Institute of Marseille, Aix-Marseille University, 13288 Marseille Cedex 9, France)

  • Erwin J. G. Peterman

    (Vrije Universiteit Amsterdam)

Abstract

Cytoplasmic dyneins drive microtubule-based, minus-end directed transport in eukaryotic cells. Whereas cytoplasmic dynein 1 has been widely studied, IFT dynein has received far less attention. Here, we use fluorescence microscopy of labelled motors in living Caenorhabditis elegans to investigate IFT-dynein motility at the ensemble and single-molecule level. We find that while the kinesin composition of motor ensembles varies along the track, the amount of dynein remains relatively constant. Remarkably, this does not result in directionality changes of cargo along the track, as has been reported for other opposite-polarity, tug-of-war motility systems. At the single-molecule level, IFT-dynein trajectories reveal unexpected dynamics, including diffusion at the base, and pausing and directional switches along the cilium. Stochastic simulations show that the ensemble IFT-dynein distribution depends upon the probability of single-motor directional switches. Our results provide quantitative insight into IFT-dynein dynamics in vivo, shedding light on the complex functioning of dynein motors in general.

Suggested Citation

  • Jona Mijalkovic & Bram Prevo & Felix Oswald & Pierre Mangeol & Erwin J. G. Peterman, 2017. "Ensemble and single-molecule dynamics of IFT dynein in Caenorhabditis elegans cilia," Nature Communications, Nature, vol. 8(1), pages 1-9, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14591
    DOI: 10.1038/ncomms14591
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms14591
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms14591?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14591. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.